Promoter-dependent disruption of genes: simple, rapid, and specific PCR-based method with application to three different yeast

PCR product-based gene disruption has greatly accelerated molecular analysis of Saccharomyces cerevisiae. This approach involves amplification of a marker gene (e.g., URA3) including its flanking regulatory (promoter and polyadenylation) regions using primers that include at their 5' ends about...

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Bibliographic Details
Published in:	Current genetics Vol. 48; no. 2; pp. 117 - 125
Main Authors:	Edlind, T.D, Henry, K.W, Vermitsky, J.P, Edlind, M.P, Raj, S, Katiyar, S.K
Format:	Journal Article
Language:	English
Published:	United States Springer Nature B.V 01-08-2005
Subjects:	Bacteria Candida albicans Candida albicans - genetics Candida glabrata Candida glabrata - genetics gene deletion Gene Targeting - methods Genes genetic recombination genetic techniques and protocols genetic transformation Genetics Hypersensitivity Methods mutagenesis Mutation polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards promoter regions Promoter Regions, Genetic promoter-dependent disruption of genes Proteins Recombination, Genetic Regulatory Sequences, Nucleic Acid - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Torulopsis glabrata Yeast Yeasts Yeasts - genetics
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