Production of recombinant antibodies in lymphoid and non-lymphoid cells

• Published in: FEBS letters Vol. 330; no. 2; pp. 111 - 113
• Main Authors: Deyev, Sergey M., Lieber, Andre, Radko, Boris V., Polanovsky, Oleg L.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 13-09-1993
• Subjects: Animals; Antibodies - genetics; Antibody engineering; CHO cell; CHO Cells; Cloning, Molecular; Cricetinae; DNA-Directed RNA Polymerases - genetics; Gene tandem expression; Humans; Mice; Phage T7 promoter; Plasmids; Recombinant Proteins - genetics; RNA, Messenger - genetics; Sp2/0 cell; Tumor Cells, Cultured; Viral Proteins; Phage T7 promoter; Antibody engineering; Sp2/0 cell; CHO cell; Gene tandem expression
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)80253-Q

A recombinant tandem of ‘chimeric’ mouse/human immunoglobulin (Ig) genes was constructed and inserted into plasmid pGEM 1 under the control of the T7 bacteriophage RNA polymerase promoter. Lymphoid (Sp2/0) and non-lymphoid (CHO) cell lines used for transfection contained in their genomes a semisynthetic gene of T7 RNA polymerase and steadily expressed this enzyme. It was shown for the first time that a stable polycystronic transcription of the Ig gene tandem occurs under the control of a single T7 phage promoter, both in lymphoid and non-lymphoid cells. Synthesis of κ-light and ε-heavy Ig chains and functionally active antibodies was observed in the above-mentioned transfected cell lines.