Genetic diversity among melon accessions (Cucumis melo) from Turkey based on SSR markers

• Published in: Genetics and molecular research Vol. 11; no. 4; pp. 4622 - 4631
• Main Authors: Kaçar, Y A, Simsek, O, Solmaz, I, Sari, N, Mendi, Y Y
• Format: Journal Article
• Language: English
• Published: Brazil 01-01-2012
• Subjects: Alleles; Cluster Analysis; Crops; Cucumis melo; Cucumis melo - genetics; Data processing; DNA, Plant - genetics; DNA, Plant - isolation & purification; Electrophoresis; Genes, Plant; Genetic distance; Genetic diversity; Genotype; Genotypes; Microsatellite Repeats; Microsatellites; Phylogeny; Plant Leaves - genetics; Polymerase chain reaction; Polymorphism, Genetic; Primers; Turkey; Vegetables; Turkey
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2012.November.29.2

Melon (Cucumis melo) is an important vegetable crop in Turkey, where it is grown in many regions; the most widely planted lines are local winter types belonging to the var. inodorous. We examined 81 melon genotypes collected from different provinces of Turkey, compared with 15 reference melon genotypes obtained from INRA/France, to determine genetic diversity among Turkish melons. Twenty polymorphic primers were used to generate the SSR markers. PCR amplification was performed and electrophoresis was conducted. SSR data were used to generate a binary matrix. For cluster analysis, UPGMA was employed to construct a clustering dendrogram based on the genetic distance matrix. The cophenetic correlation was compared with the similarity matrix using the Mantel matrix correspondence test to evaluate the representativeness of the dendrogram. A total of 123 alleles were amplified using the 20 SSR primer sets. The number of alleles detected by a single primer set ranged from 2 to 12, with an average of 6.15. The similarity ranged from 0.22 to 1.00 in the dendrogram developed from microsatellite analysis. Based on this molecular data, we concluded that genetic diversity among these Turkish accessions is relatively high.