Transcriptional regulation of the purine de novo synthesis gene Prat in Drosophila melanogaster

• Published in: Gene Vol. 518; no. 2; pp. 280 - 286
• Main Authors: Merzetti, Eric, Hackett, Joanne M., Clark, Denise V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-04-2013
• Subjects: Adenosine Triphosphatases - genetics; Amidophosphoribosyltransferase - genetics; Amidophosphoribosyltransferase - metabolism; Animals; Autoantigens - genetics; Base Sequence; BEAF-32; cell proliferation; diminutive; Distal-less; dMyc; DNA; DNA-Binding Proteins - genetics; Dref; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Female; Gene Expression Regulation; genes; germ cells; Homeodomain Proteins - genetics; imaginal discs; Imaginal Discs - metabolism; Mi-2; Purines - biosynthesis; Sequence Alignment; transcription (genetics); Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic; diminutive; Scs; PCNA; Mi-2; S2 cells; BEAF-32; ChIP; Distal-less; DREF; PRAT; Ct; MEME; DRE; RT-PCR; TESS; qPCR; dMyc
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2013.01.024

The first step of the purine de novo synthesis pathway is catalyzed by amidophosphoribosyltransferase (E.C.2.4.2.14) which is encoded by two Prat genes in D. melanogaster, Prat and Prat2. Prat is a retrogene duplication of Prat2, where each gene has a distinct expression pattern. Prat transcription is restricted to proliferating tissues such as imaginal discs and the female germ line. Three conserved putative DNA replication-related element binding factor (DREF) sites lie upstream of the Prat coding region. These elements are upstream of many genes important in cell proliferation. We have found that DREF binds directly upstream of Prat and that the DRE sites associated with its activity are necessary for Prat expression; furthermore, we have determined that a second cis-acting element is present upstream of the Prat gene. Finally, the genes Distal-less, Mi-2 and dMyc, which influence Dref activity, do not appear to affect Prat transcription.
► Links are found between a purine synthesis gene and its regulation in Drosophila. ► The cell-cycle-related DREF transcription factor controls Prat expression. ► The central of 3 DREF binding sites has the greatest influence on expression. ► BEAF-32 is also associated with Prat upstream sequence. ► Dref regulators, dMyc, Mi-2, Dll and BEAF-32 are not limiting for Prat expression.