Conjugation of Active Iron Superoxide Dismutase to Nanopatterned Surfaces

• Published in: IEEE transactions on nanobioscience Vol. 11; no. 2; pp. 176 - 180
• Main Authors: Tellechea, E., Cornago, I., Ciaurriz, P., Moran, J. F., Asensio, A. C.
• Format: Magazine Article
• Language: English
• Published: United States IEEE 01-06-2012; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Azo Compounds - chemistry; Biosensing Techniques - instrumentation; Electrostatics; Enzymes; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Fabaceae - enzymology; Free radicals; Gold; Gold - chemistry; Hydrogen Peroxide - chemistry; Iron; Iron superoxide dismutase; Microscopy; Microscopy, Fluorescence; nanopattern; nanosensor; Nanostructures; Nanostructures - chemistry; Nanotechnology - methods; Plant Proteins - chemistry; Plant Proteins - metabolism; Protein Binding; Proteins; Silicon Dioxide - chemistry; Static Electricity; superoxide; superoxide dismutase (SOD); Superoxide Dismutase - chemistry; Superoxide Dismutase - metabolism; Surface Properties
• ISSN: 1536-1241; 1558-2639
• DOI: 10.1109/TNB.2012.2194742

Superoxide dismutase enzymes (SODs) are an essential part of the first line of cellular defense system against free radicals species. They catalyze the dismutation of superoxide radicals into oxygen and hydrogen peroxide. Although several studies have examined the attachment of superoxide dismutases to nanoparticles and nanostructures, never has been used a member of the Fe/MnSOD family. In this study, the behavior of plant origin FeSOD enzyme on three different nanopatterned surfaces was investigated as a function of covalent and electrostatic binding. Fluorescence microscopy was used to demonstrate that the protein is attached only to the gold layer. We also examined the activity of SOD by a colorimetric assay, and we have shown that the enzyme remains active after attachment to the three different surfaces under both kind of binding (electrostatic and covalent). This methodology could be useful for those who want to functionalize nanostructures with a SOD enzyme and test the activity. This process could be of great interest for the development of peroxynitrite and superoxide biosensors.