Microbial transformation of Astragalus sapogenins using Cunninghamella blakesleeana NRRL 1369 and Glomerella fusarioides ATCC 9552

Microbial transformation of Astragalus sapogenins using Cunninghamella blakesleeana NRRL 1369 and Glomerella fusarioides ATCC 9552

•Microbial transformations of Astragalus saponins were investigated.•Two fungi were used as whole cell systems.•Each organism led to characteristic transformations with different substrates.•Eight molecules are introduced to the literature for the first time. The microbial transformation of Astragal...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Vol. 115; pp. 29 - 34
Main Authors: Bedir, Erdal, Kula, Ceyda, Öner, Özlem, Altaş, Melis, Tağ, Özgür, Öngen, Gaye
Format: Journal Article
Language:English
Published: Elsevier B.V 01-05-2015
Subjects:
Astragalus
Astragenol
Cycloastragenol
Cyclocanthogenol
Microbial transformation
Microbial transformation
Cyclocanthogenol
Cycloastragenol
Astragalus
Astragenol
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Microbial transformations of Astragalus saponins were investigated.•Two fungi were used as whole cell systems.•Each organism led to characteristic transformations with different substrates.•Eight molecules are introduced to the literature for the first time. The microbial transformation of Astragalus sp. derived sapogenins, namely cycloastragenol, astragenol and cyclocanthogenol, by Cunninghamella blakesleeana NRRL 1369 and Glomerella fusarioides ATCC 9552 were investigated. The unique enzyme system of both fungi resulted hydroxylation, cyclization, dehydrogenation and oxidation reactions. Structures of the new metabolites were elucidated by 1-D (1H, 13C, NOESY), 2-D NMR (DQF-COSY, HMBC, HMQC, NOESY) and HR-MS analyses.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2015.01.013