Effect of Exogenous Anti-Müllerian Hormone Treatment on Cryopreserved and Transplanted Mouse Ovaries

• Published in: Reproductive sciences (Thousand Oaks, Calif.) Vol. 23; no. 1; p. 51
• Main Authors: Kong, Hyun Sun, Kim, Seul Ki, Lee, Jaewang, Youm, Hye Won, Lee, Jung Ryeol, Suh, Chang Suk, Kim, Seok Hyun
• Format: Journal Article
• Language: English
• Published: United States 01-01-2016
• Subjects: Animals; Anti-Mullerian Hormone - administration & dosage; Anti-Mullerian Hormone - pharmacology; Apoptosis - drug effects; Cryopreservation; Dose-Response Relationship, Drug; Female; Follicle Stimulating Hormone - blood; Mice; Ovarian Follicle - drug effects; Ovary - drug effects; Ovary - transplantation; Vitrification; anti-Müllerian hormone; fertility preservation; ovarian tissue; vitrification; transplantation
• ISSN: 1933-7205
• DOI: 10.1177/1933719115594021

Follicle loss occurs after ovary cryopreservation and transplantation. To preserve the follicle pool of cryopreserved or grafted ovaries, anti-Müllerian hormone (AMH), which inhibits ovarian follicle recruitment, was used in a mouse model. In experiment 1, ovaries were vitrified warmed with different doses of AMH (0, 5, 15, or 45 μg/mL) supplementation. In experiment 2, AMH (0, 50, 250, and 1250 μg/mL) was injected into mice before and/or after cryopreserved ovary autotransplantation, and the recipients remained for 7 or 28 days after grafting. Ovaries were evaluated by follicle morphology, density, and apoptosis ratio. Additionally, serum follicle-stimulating hormone was measured in experiment 2. Significantly decreased follicle apoptosis were detected in AMH-treated groups when compared to the control ovaries in experiment 1, meanwhile no positive effect of exogenous AMH was found in experiment 2. Thus, we suggest AMH supplementation during ovary vitrification warming has beneficial effect on reducing follicle apoptosis.