linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs)

• Published in: Theoretical and applied genetics Vol. 95; no. 8; pp. 1289 - 1299
• Main Authors: Gilpin, B.J, McCallum, J.A, Frew, T.J, Timmerman-Vaughan, G.M
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-12-1997; Berlin Springer Nature B.V
• Subjects: amplified fragment length polymorphism; Biological and medical sciences; chromosome mapping; chromosomes; Classical genetics, quantitative genetics, hybrids; complementary DNA; Fundamental and applied biological sciences. Psychology; genbank/aa427337; genbank/aa427338; genbank/aa427339; genbank/aa430902; genbank/aa430903; genbank/aa430904; genbank/aa430905; genbank/aa430906; genbank/aa430907; genbank/aa430908; genbank/aa430909; genbank/aa430910; genbank/aa430911; genbank/aa430912; genbank/aa430913; genbank/aa430914; genbank/aa430915; genbank/aa430916; genbank/aa430917; genbank/aa430918; genbank/aa430919; genbank/aa430920; genbank/aa430921; genbank/aa430922; genbank/aa430923; genbank/aa430924; genbank/aa430925; genbank/aa430926; genbank/aa430927; genbank/aa430950; genbank/aa430951; genetic markers; Genetics of eukaryotes. Biological and molecular evolution; genome; Genomes; linkage groups; loci; molecular sequence data; nucleotide sequences; Peas; Pisum sativum; Pteridophyta, spermatophyta; random amplified polymorphic DNA technique; restriction fragment length polymorphism; sequence tagged sites; Vegetals; Genetic mapping; Genome organization; Linkage; Genetic marker; Nucleotide sequence; Chromosome; Grain legume; Leguminosae; Gene; Dicotyledones; Pisum sativum; Angiospermae; Spermatophyta; Expressed sequence tag
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050695

A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar 'Primo' and the blue-pea breeding line 'OSU442-15'. This linkage map consists of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor loci, the 'Primo' x 'OSU442-15' map has been related to other pea linkage maps. A feature of the map is the incorporation of 29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed for single-copy sequences. Eleven polymorphic STSs were developed.