Immunohistochemical evaluation of Langerhans cells in oral lichen planus and oral lichenoid lesions

• Published in: Archives of oral biology Vol. 124; p. 105027
• Main Authors: Ferrisse, Túlio Morandin, de Oliveira, Analú Barros, Palaçon, Mariana Paravani, da Silveira, Heitor Albergoni, Massucato, Elaine Maria Sgavioli, de Almeida, Luciana Yamamoto, Léon, Jorge Esquiche, Bufalino, Andreia
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-04-2021
• Subjects: Dendritic cells; Dentistry; Humans; Immunohistochemistry; Langerhans Cells; Lichen Planus, Oral; Lichenoid Eruptions; Multivariate analyses; Oral lichen planus; Regression model; Oral lichen planus; Immunohistochemistry; Multivariate analyses; Langerhans cells; Dendritic cells; Regression model
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2020.105027

•Oral lichen planus can be considered CD207high comparing to oral lichenoid lesions.•Oral inflammatory fibrous hyperplasia can be a more appropriate control group.•Multivariate analyses are the better choice to evaluate immunohistochemical markers.•Immunomediated diseases showed a clear dependency of Langerhans cells.•Regression models can be use by others authors to estimates the Langerhans cells.
the aim of this study was to evaluate the density of Langerhans cells in oral lichen planus (OLP) and oral lichenoid lesions (OLL).
14 cases of OLP, 15 cases of OLL and 14 cases of oral inflammatory fibrous hyperplasia (OIFH), were selected for immunohistochemical analysis of CD1a, CD207 and S100 expression. The OIFH group was subdivided according to the presence (OIFHL n = 14) or absence (OIFHNL n = 14) of lichenoid inflammatory infiltrate. Positive cells were counted in intraepithelial and subepithelial areas. Results were analyzed by multivariate comparative analysis, correlation analysis, linear regression models and Student’s T-test.
A significantly higher amount of CD207+ cells in OLL vs OLP was observed (p = 0.015). The prevailing reticular pattern observed was CD207high for OLP (p = 0.0329). A statistically significant difference in the expression of CD1a and CD207 was observed for intraepithelial vs subepithelial areas (p = 0.024 and p=0.015, for CD1a and CD207, respectively). Significant correlations were also observed between the expression of CD1a + and CD207+ cells in the pathogenesis of OLP and OLL.
High levels of CD207+cells in OLP compared with OLL may help explain the differences in the immunopathogenesis of both diseases. Additionally, CD1a + and CD207+ cells appear to be more essential to immunopathogenesis of OLL than to the pathogenesis of OLP.