Immunoexpression of DNA base excision repair and nucleotide excision repair proteins in ameloblastomas, syndromic and non-syndromic odontogenic keratocysts and dentigerous cysts

• Published in: Archives of oral biology Vol. 110; p. 104627
• Main Authors: Santos, Hellen Bandeira de Pontes, Morais, Everton Freitas de, Cavalcante, Roberta Barroso, Nogueira, Renato Luiz Maia, Nonaka, Cassiano Francisco Weege, Souza, Lélia Batista de, Freitas, Roseana de Almeida
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-02-2020
• Subjects: Ameloblastoma - genetics; Dentigerous Cyst - genetics; Dentistry; DNA; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism; DNA-Binding Proteins - metabolism; Gene Expression; Humans; Immunohistochemistry; Odontogenic cyst; Odontogenic Cysts - genetics; Odontogenic tumour; X-ray Repair Cross Complementing Protein 1 - metabolism; Immunohistochemistry; Odontogenic cyst; DNA repair; Odontogenic tumour
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2019.104627

•This is the first study about DNA BER and NER proteins in odontogenic lesions.•Overexpression of APE-1, XRCC-1 and XPF was found in solid AMEs, NSOKCs, and SOKCs.•APE-1, XRCC-1 and XPF may regulate events related to a more aggressive behavior.•APE-1 expression may be synergistic with XRCC-1 and XPF in all studied lesions.•These proteins may be involved in the development of odontogenic lesions.
To evaluate the immunoexpression of DNA base excision repair (BER) [apurinic/apyrimidinic endonuclease 1 (APE-1), X-ray repair cross complementing 1 (XRCC-1)] and nucleotide excision repair (NER) [xeroderma pigmentosum complementation group (XPF)] proteins in benign epithelial odontogenic lesions with different biological behaviors.
Thirty solid ameloblastomas, 30 non-syndromic odontogenic keratocysts (NSOKCs), 29 syndromic odontogenic keratocysts (SKOCs), 30 dentigerous cysts (DCs) and 20 dental follicles (DFs) were evaluated quantitatively for APE-1, XRCC-1 and XPF through immunohistochemistry.
Nuclear expression of APE-1 was significantly higher in NSOKCs, SOKCs, and ameloblastomas in comparison to DCs (p < 0.001). Nuclear expression of XRCC-1 was higher in NSOKCs and SOKCs than in DCs (p < 0.05). At the nuclear level, XPF expression was higher in NSOKCs and SOKCs than in DCs and ameloblastomas (p < 0.05). A statistically significant higher expression of APE-1 (nuclear), XRCC-1 (nuclear), and XPF (nuclear and cytoplasmic) was found in all odontogenic lesion samples as compared to DFs (p < 0.05). For all lesions, there was a positive correlation between nuclear expression of APE-1 and XRCC-1 or XPF (p < 0.05).
Our results suggest a potential involvement of APE-1, XRCC-1 and XPF proteins in the pathogenesis of benign epithelial odontogenic lesions, especially in those with more aggressive biological behavior, such as ameloblastomas, NSOKCs, and SOKCs. We also showed that the expression of APE-1 was positively correlated with the nuclear expression of XRCC-1 and XPF, which may suggest an interaction between the BER and NER pathways in all odontogenic lesions studied herein.