Oxidative stress in the hybrid fish jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) exposed to Roundup Original

• Published in: Chemosphere (Oxford) Vol. 185; pp. 445 - 451
• Main Authors: de Moura, Fernando Rafael, Brentegani, Kamila Ribeiro, Gemelli, Aline, Sinhorin, Adilson Paulo, Sinhorin, Valéria Dornelles Gindri
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-2017
• Subjects: Acetylcholinesterase - metabolism; Animals; Antioxidants; Antioxidants - metabolism; Catfishes - metabolism; Gills - metabolism; Glycine - analogs & derivatives; Glyphosate; Herbicides - toxicity; Hybrid fish; Liver - metabolism; Muscles - metabolism; Oxidative Stress - drug effects; Thiobarbituric Acid Reactive Substances - metabolism; Toxicity; Antioxidants; Hybrid fish; Toxicity; Glyphosate
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.07.030

The aim of this study was to investigate the effects of Roundup Original®, a glyphosate-based herbicide, against biochemical parameters including thiobarbituric acid-reactive substances (TBARS), protein carbonyl, enzymatic and nonenzymatic antioxidant responses and acetylcholinesterase (AChE) of jundiara fish (Leiarius marmoratus × Pseudoplatystoma reticulatum) at a sublethal concentration of 1.357 mg L−1. Fish exposed to the herbicide for different periods (6–96 h) showed a significant increase of both hepatic and muscular TBARS and protein carbonyl. Enzymatic antioxidant activity was decreased in the liver and brain after 48 h of exposure. Glutathione-S-tranferase (GST) had its levels raised in the brain and gills, probably as a toxicity event response. Non protein thiols (GSH) demonstrated a reduction after 6 and 24 h of exposure in the hepatic tissue, followed by an increase at 48 and 96 h in the same tissue. GSH brain levels, however, increased only after 96 h. AChE activity in muscle decreased for all the times tested (26.5, 45, 38 and 14% for 6, 24, 48 and 96 h respectively), but only at 96 h (34%) in the brain. We found that Roundup Original® is able to trigger important changes in the biochemical parameters tested, showing it can be a potential threat for the health and survival of fish in the environment.
•We examined the effects of Roundup Original® herbicide on oxidative stress parameters in a hybrid fish.•Our results confirm that the glyphosate-based herbicide promotes oxidative stress in these fish.•Reduction of AChE activity can be used as a suitable indicator of herbicidal toxicity.