Characterization of extended-spectrum and CMY-2 ß-lactamases, and associated virulence genes in Escherichia coli from food of animal origin in México

• Published in: British food journal (1966) Vol. 120; no. 7; pp. 1457 - 1473
• Main Authors: Barrios-Villa, Edwin, Cortés-Cortés, Gerardo, Lozano Zarain, Patricia, Romero-Romero, Sergio, Lara Flores, Norarizbeth, Estepa, Vanesa, Somalo, Sergio, Torres, Carmen, Rocha-Gracia, Rosa del Carmen
• Format: Journal Article
• Language: English
• Published: Bradford Emerald Publishing Limited 07-08-2018; Emerald Group Publishing Limited
• Subjects: Animals; Antibiotics; Antimicrobial agents; Bacteria; Cefotaxime; Cephalosporins; Chickens; Drug resistance; E coli; Enzymes; Escherichia coli; Food chains; Food products; Food safety; Gene sequencing; Genes; Genotype & phenotype; Laboratories; Multilocus sequence typing; Mutation; Phylogenetics; Phylogeny; Pork; Supermarkets; Virulence; β Lactamase; Mexico; CMY-2; ESBL; Pork meat; Virotype; Chicken meat
• ISSN: 0007-070X; 1758-4108
• DOI: 10.1108/BFJ-02-2018-0104

Purpose
Broad-spectrum cephalosporin resistance is rapidly increasing in Escherichia coli, representing a food safety problem. The purpose of this paper is to characterize eight extended-spectrum-ß-lactamase (ESBL) and acquired AmpC ß-lactamase-producing E. coli isolates and virotypes associated, obtained from chicken and pork food samples in Puebla, Mexico.
Design/methodology/approach
Samples (36 from chicken and 10 from pork) were cultured on Levine agar plates supplemented with cefotaxime (2 mg/L) for isolation of cefotaxime-resistant (CTXR) E. coli. CTXR-E. coli isolates were detected in 33 of 46 samples (72 percent), and one isolate/sample was characterized (28 from chicken and 5 from pork), for ESBL production, phylogenetic group, sequence typing, resistance and virulence genes by PCR and sequencing.
Findings
Results showed 16 ESBL-E. coli (35 percent) (12/16 belonging to phylogroup B1) and 8 CMY-2-E. coli (17 percent). ESBL detected were as follows (number of isolates): CTX-M-2 (8); CTX-M-1 (2); CTX-M-15 (1); SHV-2a (4) and TEM-52c (1). In total, 20 different sequence types (STs) were identified among the ESBL- or CMY-2-producing E. coli strains, which included four new ones. The CTX-M-15 β-lactamase was detected in one E. coli ST617-ST10 Cplx-B1 strain that also carried ibeA gene. One CMY-2-positive strain of lineage ST224-B2 was detected and it carried the qnrA1 gene.
Originality/value
In this study, a ST131-based virotyping scheme for strains from food of animal origin was established since this kind of strains constitutes an important vehicle of virulent ESBL- and CMY-2-producing E. coli isolates, which could be transmitted to humans by direct contact or through the food chain.