From molecules to ecosystems: Insights into a network of interactions for a Chagas disease outbreak using Triatoma brasiliensis as natural samplers

• Published in: Acta tropica Vol. 251; p. 107107
• Main Authors: Almeida, Carlos E., Máximo, Milena M., Pires-Silva, Dayane, Takiya, Daniela M., Valença-Barbosa, Carolina, Viana, Maria C., Reigada, Carolina, Iñiguez, Alena M., Harry, Myriam, Folly-Ramos, Elaine
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-03-2024
• Subjects: Chagas disease; Ecohealth; Environmental health; Human health; T. cruzi reservoirs; Trypanosoma cruzi vectors; Chagas disease; Trypanosoma cruzi vectors; Human health; Ecohealth; T. cruzi reservoirs; Environmental health
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2023.107107

•Significant triatoma brasiliensis-parasite-host-environment interactions provided crucial insights into Chagas disease dynamics.•We showed that anthropogenic changes facilitate trypanosoma cruzi circulation.•By incorporating eco-biological interactions with local community issues, our study adopts a comprehensive and integrated approach.
Exploring the dynamics of disease transmission involves an understanding of complex interactions within the eco-epidemiologic framework. In the context of Chagas disease (CD), elements are mainly represented by the interactions among the pathogen, insect vector, host, humans and the environment. We performed quantitative and qualitative analyses on a dataset derived from 98 Triatoma brasiliensis infected by trypanosomatids, which were linked to a CD outbreak in the semi-arid region of northeastern Brazil. We extracted invertebrate-derived DNA (iDNA) from these insects, comprising 18 populations around the outbreak area, each indicative of various strata of anthropogenic influence. Food source (FS) diversity, representing potential parasite reservoirs, was determined through mitochondrial gene (cyt b) sequencing of vertebrates, and parasite genotyping was accessed using fluorescent amplified fragment barcodes (FFLB) of trypanosomatids. We also assessed the residents' awareness of breeding sites for CD vectors in the inspected houses. The quantification of Trypanosoma cruzi was estimated via real-time PCR and is denominated here as the average parasite load (PL) per insect (T. cruzi/intestinal unit). We aimed to address vector-parasite-host-environment interactions that were discussed based on their significance among the components. Notably, among the significant interactions, we observed that the PL in the insects was significantly influenced by FS. Infected insects that fed on the classic reservoir, Didelphis albiventris, and Galea spixii exhibited higher PLs, compared to those that fed on Kerodon rupestris (p < 0.04)—a primary host. While D. albiventris is already recognized as a synanthropic species, we propose that G. spixii may also be undergoing a synanthropic process. Conversely, domestic cats are frequently identified as FS in infected insects from the sylvatic environment, suggesting a possible change in their behavior towards a wild state. Therefore, we propose that neglected anthropogenic actions have facilitated the reciprocal (sylvatic-peridomestic) circulation of T. cruzi—especially noted for TcI because it was predominant in insects found in peridomestic environments. Residents are often unaware of the existence of insect breeding grounds near their homes, particularly when it involves the storage of materials without planning for use, such as piles of tiles, bricks and wood. Although indirect inferences about the interaction among vector-parasite-host-environment are still incipient, we highlight the potential use of vectors as natural samplers of biological and ecological components in transmitting the disease.
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