Interstitial collagenase MMP-1 and EMMPRIN in cell lines and in clinical specimens of cervical squamous cell carcinoma

• Published in: Molecular biology reports Vol. 48; no. 10; pp. 6879 - 6886
• Main Authors: Solovyeva, Nina I., Timoshenko, Olga S., Kugaevskaya, Elena V., Gureeva, Tatyana A.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Basigin - genetics; Basigin - metabolism; Biomedical and Life Sciences; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Cervical cancer; Cervix; Collagen; Collagenase; Densitometry; Female; Gene expression; Gene Expression Regulation, Neoplastic; Histology; Humans; Interstitial collagenase; Invasiveness; Life Sciences; Matrix metalloproteinase; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 1 - metabolism; Metalloproteinase; Metastases; Morphology; Original Article; Polymerase chain reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Squamous cell carcinoma; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology; HPV18; EMMPRIN; Cervical squamous cell carcinoma; MMP-1; HPV16
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06689-z

Background
The aim of this study was to elucidate the features of the expression of matrix metalloproteinases inducer—EMMPRIN (EMN) and matrix metalloproteinase 1 (MMP-1) in cell lines and in clinical samples of cervical squamous cell carcinoma (SCC).
Material and methods
The study was carried out using RT-PCR, densitometry and immunohistochemical studies (IHC) on commercial cell lines Siha, Caski, transformed with HPV16; HeLa, and C33A transformed with HPV18, line C33A without HPV, and in clinical samples of SCC and morphologically normal tissue adjacent to the tumor.
Results
The data obtained indicate that the expression of mRNA EMN and MMP-1 occurs in all cell lines at different levels. HPV type and number of genes copies had no effect on expression degree both EMN and MMP-1. Gene expression of EMN and MMP-1 has been investigated in tumor and normal tissues. MMP-1 expression in tumor tissue in SCC, as a rule, has been significantly increased (2–6 times) compared to normal tissue. It was found in 90% of tumor samples. It is known, that MMP-1 promotes the development of invasive and metastatic processes. EMN expression was lower in the tumor tissue than in normal tissue in most cases. An increase in EMN expression was noted only in some cases of SCC.
Conclusion
The data obtained indicate that MMP-1 can serve as a marker of the invasive potential of SCC. EMN, apparently, is not a major factor responsible for MMP-1 expression in SCC. Data are important for understanding the process of tumor development and may have prognostic value for the patient.