Expression of GAD65/67 and VGLUT2 in Mediobasal Nuclei of Rat Hypothalamus during Aging

• Published in: Bulletin of experimental biology and medicine Vol. 176; no. 2; pp. 232 - 234
• Main Authors: Anfimova, P. A., Pankrasheva, L. G., Emanuilov, A. I., Moiseev, K. Yu, Maslyukov, P. M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2023; Springer Nature B.V
• Subjects: Age groups; Aging; Aging - genetics; Animals; Biogerontology; Biomedical and Life Sciences; Biomedicine; Cell Biology; Conflicts of interest; Enzymes; Glutamate decarboxylase; Glutamate Decarboxylase - genetics; Glutamate Decarboxylase - metabolism; Glutamic acid transporter; Homeostasis; Hypothalamus; Hypothalamus (ventromedial); Hypothalamus - metabolism; Internal Medicine; Laboratory Medicine; Male; Metabolism; Neurons; Neurons - metabolism; Nuclei; Older people; Pathology; Rats; Software; Western blotting; γ-Aminobutyric acid; ageing; GAD65/67; VGLUT2; hypothalamus; Western blotting
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-024-06001-6

The expression of glutamate decarboxylase GAD65/67, an enzyme of GABA synthesis, and vesicular glutamate transporter 2 (VGLUT2) in the arcuate, dorsomedial, and ventromedial nuclei of the hypothalamus of young (3 months), adult (12 months), and old male rats (24 months) was studied by Western blotting. In old rats, an increase in the expression of GAD65/67 in the arcuate and dorsomedial, VGLUT2 in the arcuate, dorsomedial, and ventromedial nuclei was observed. Thus, an increase in opposite processes of inhibition and excitation is observed in the hypothalamic nuclei during aging.