Transfer of Cornus florida and C. kousa Simple Sequence Repeats to Selected Cornus (Cornaceae) Species

• Published in: Journal of the American Society for Horticultural Science Vol. 135; no. 3; pp. 279 - 288
• Main Authors: Wadl, Phillip A, Wang, Xinwang, Moulton, John K, Hokanson, Stan C, Skinner, John A, Rinehart, Timothy A, Reed, Sandra M, Pantalone, Vincent R, Trigiano, Robert N
• Format: Journal Article
• Language: English
• Published: 01-05-2010
• Subjects: Cornus; Cornus florida; Cornus kousa; cross pollination; genetic markers; genetic polymorphism; genetic variation; internal transcribed spacers; interspecific hybridization; loci; microbial systematics; microsatellite repeats; molecular sequence data; ornamental trees; phylogeny; sequence analysis; species differences
• ISSN: 0003-1062; 2327-9788
• DOI: 10.21273/jashs.135.3.279

Cross-species transferability of simple sequence repeats (SSRs) is common and allows SSRs isolated from one species to be applied to closely related species, increasing the use of previously isolated SSRs. The genus Cornus consists of 58 species that are ecologically and economically important. SSRs have previously been isolated from C. florida and C. kousa. In this study, 36 SSRs were tested on taxa from 18 Cornus species and hybrids for cross-species transferability and genetic diversity was calculated for each locus using polymorphism information content (PIC). Cross-species transferability of SSR loci was higher in more closely related species and PIC values were high. Evidence was found for conserved primer sites as determined by the amplification of SSR loci in the taxa examined. Polymerase chain reaction products were cloned and sequenced for three SSR loci (CF48, CF59, and CF124) and all individuals sequenced contained the appropriate repeat. Phylogenetic relationships of 14 Cornus species were inferred using nucleotide sequences of SSR locus CF48. The most parsimonious tree resulting from this analysis was in concordance with phylogenies based on matK and internal transcribed spacer sequences. The SSR loci tested in this study will be useful in future breeding, population, and genetic studies within Cornus.