Molecular identification and detection of Wohlfahrtia magnifica in ovine vulvar myiasis in Gansu, China

• Published in: Tropical animal health and production Vol. 51; no. 8; pp. 2629 - 2634
• Main Authors: Li, Youquan, Li, Xuan, Liu, Junlong, Liu, Aihong, Guo, Pengfei, Han, Yuan, Shang, Youjun, Guan, Guiquan, Liu, Zhijie, Liu, Guangyuan, Luo, Jianxun, Yin, Hong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-11-2019; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; China - epidemiology; Economic impact; Female; Insect Proteins - analysis; Larva - genetics; Larva - growth & development; Larva - physiology; Larvae; Life Sciences; Myiasis; Myiasis - epidemiology; Myiasis - parasitology; Myiasis - veterinary; Ovis aries; Phylogeny; Polymerase chain reaction; Prevalence; Risk reduction; Sarcophagidae - genetics; Sarcophagidae - growth & development; Sarcophagidae - physiology; Sequence Analysis, DNA - veterinary; Sheep; Sheep Diseases - epidemiology; Sheep Diseases - parasitology; Short Communications; Veterinary Medicine/Veterinary Science; Vulvar Diseases - epidemiology; Vulvar Diseases - parasitology; Vulvar Diseases - veterinary; White gene; Wohlfahrtia; Zoology; China; Gansu China; Gansu; Sheep; Vulvar myiasis; Identification; PCR; Wohlfahrtia magnifica
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-019-01925-y

Traumatic myiasis causes substantial economic losses to farmers worldwide. In the present study, six flocks of sheep (2261 sheep) were investigated in Gansu, China, and 207 of 552 larvae were genetically characterized based on three genes, including
cyt
b,
EF-1α
, and
white
gene, by polymerase chain reaction and sequence analysis. A survey of sheep in China revealed that the prevalence of vulvar myiasis of six sheep flocks was 5.00% (11/220, Flock1), 4.85% (10/206, Flock2), 4.50% (9/200, Flock3), 5.00% (15/300, Flock4), 4.68% (15/320, Flock5), 0% (0/1015, Flock6), respectively. The sequence and phylogenetic analysis showed that only
Wohlfahrtia magnifica
was detected in the field samples. This is the first report of ovine vulvar myiasis caused by
W. magnifica
in Gansu, China. Some prophylactic measures are strongly recommended to reduce the risk of sheep acquiring traumatic myiasis in Gansu, China.