Genetic variability of pig and human rotavirus group A isolates from Slovakia

• Published in: Archives of virology Vol. 165; no. 2; pp. 463 - 470
• Main Authors: Salamunova, S., Jackova, A., Csank, T., Mandelik, R., Novotny, J., Beckova, Z., Helmova, L., Vilcek, S.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-02-2020; Springer Nature B.V
• Subjects: Animals; Antigens, Viral - genetics; Biomedical and Life Sciences; Biomedicine; Brief Report; Capsid Proteins - genetics; Feces - virology; Genetic analysis; Genetic variability; Genetic Variation; Genotype; Genotypes; Humans; Infectious Diseases; Medical Microbiology; Phylogeny; Polymerase chain reaction; Rectum; Rectum - virology; Reverse Transcriptase Polymerase Chain Reaction; Rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus - isolation & purification; Rotavirus Infections - veterinary; Rotavirus Infections - virology; Sequence Analysis, DNA; Slovakia; Strains (organisms); Sus scrofa; Swine; Swine Diseases - virology; Virology; Slovakia
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-019-04504-6

The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.