Dendrobium officinale polysaccharides improved reproductive oxidative stress injury in male mice treated with cyclophosphamide

• Published in: Environmental science and pollution research international Vol. 30; no. 48; pp. 106431 - 106441
• Main Authors: Mu, Yi, Che, Bangwei, Tang, Kaifa, Zhang, Wenjun, Xu, Shenghan, Li, Wei, He, Jun, Liu, Miao, Chen, Peng, Zhong, Siwen, Li, Guangyu
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2023; Springer Nature B.V
• Subjects: 8-Hydroxydeoxyguanosine; Animals; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bcl-2 protein; c-Kit protein; Cyclophosphamide; Cyclophosphamide - toxicity; Dendrobium; Dendrobium officinale; Down-regulation; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Follicle-stimulating hormone; Gene expression; Gonadal Steroid Hormones; Hormones; Immunoregulation; Injury analysis; Injury prevention; Male; Males; Mice; Nitric oxide; Oxidative Stress; p53 Protein; Polymerase chain reaction; Polysaccharides; Polysaccharides - pharmacology; Proteins; Reproductive disorders; Research Article; RNA, Messenger; RNA-directed DNA polymerase; Saccharides; Seeds; Sertoli cells; Sex hormones; Sperm; Sperm Motility; Testes; Waste Water Technology; Water Management; Water Pollution Control; polysaccharides; Oxidative stress; Cyclophosphamide; Antioxidant; Male infertility; Dendrobium officinale polysaccharides
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-29874-y

Polysaccharides from
Dendrobium officinale
polysaccharides (
DOP
s) are the main bioactive components of
Dendrobium officinale
, which have the functions of antioxidation and immune regulation. However, it is not clear whether DOPs have any effect on the prevention of reproductive disorders induced by oxidative stress. The purpose of this study was to explore the protective effect of DOPs on reproductive oxidative stress injury in male mice and its possible mechanism. In this study, the mouse model of reproductive injury was established by intraperitoneal injection of cyclophosphamide (CTX). The reproductive function was evaluated by relative testicular mass, sperm parameters, and sex hormone levels. The oxidative stress level of male mice with reproductive injury treated with DOPs was analyzed by the levels of 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO) in sperm. The expression of follicle-stimulating hormone receptor (
FSHR
) mRNA, androgen-binding (
ABP
) mRNA, and
c-kit
mRNA was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to explore its mechanism. After CTX administration, the sperm density, sperm motility, normal sperm morphology, and sex hormone levels in mice were significantly lower than those in the control group (
P
< 0.05). At the same time, the expression of p53 protein was upregulated, and the expression of Bcl-2 protein was downregulated (
P
< 0.05). In addition, the expression of
FSHR
and
ABP
mRNA on Sertoli cells was also significantly inhibited (
P
< 0.05). DOPs can effectively reduce the oxidative stress injury of testicular tissue. After DOP treatment, the sperm quality and sex-related hormone levels of mice were significantly improved and positively correlated with the dose of DOPs (
P
< 0.05). Administration of DOPs can reduce the damage caused by oxidative stress by reducing the level of oxidative stress, improving the hormone environment in testes, and regulating the expression of specific genes in Sertoli cells and spermatogenic cells.