Serum Elemental Analysis of Type 2 Diabetes Patients Using SRXRF

• Published in: Biological trace element research Vol. 200; no. 4; pp. 1485 - 1494
• Main Authors: Rao, N. Srinivasa, Raju, G. J. Naga, Tiwari, M. K., Naidu, B. G., Sarita, P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-04-2022; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Calcium; Chromium; Cobalt; Copper; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2; Fluorescence; Homeostasis; Humans; India; Insulin; Iron; Lead; Life Sciences; Manganese; Nickel; Nutrition; Oncology; Oxidative stress; Radiation; Selenium; Serum; Synchrotron radiation; Synchrotrons; Trace Elements - analysis; Uptake; X-ray fluorescence; X-Rays; Zinc; India; Type 2 diabetes; Elemental concentrations; SRXRF; Diabetes mellitus
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-021-02762-7

A total of 158 serum samples of newly diagnosed type 2 diabetes patients and control subjects were analyzed using Synchrotron Radiation X-ray Fluorescence (SRXRF) technique. The microprobe XRF beam line-16 of Indus-2 synchrotron radiation facility at Raja Ramanna Centre for Advanced Technology (RRCAT), Indore, India, was used to identify and quantify the elements K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br, Rb, Sr, and Pb. A significant decrease in the mean concentrations of K, Ca, Ti, Cr, Mn, Ni, Zn, and As and an increase in the concentrations of V, Fe, Co, Cu, Se, and Pb were observed in the serum samples of the patient group when compared to the control group. It is hypothesized that the observed alterations in the elemental concentrations might have led to ineffective uptake of insulin and have interfered with glucose homeostasis by either directly or indirectly causing oxidative stress.