Definition of Liquid and Powder Cellulase Formulations Using Domestic Wastewater in Bubble Column Reactor

• Published in: Applied biochemistry and biotechnology Vol. 190; no. 1; pp. 113 - 128
• Main Authors: Libardi, Nelson, Soccol, Carlos Ricardo, Tanobe, Valcineide O. A., Vandenberghe, Luciana Porto de Souza
• Format: Journal Article
• Language: English
• Published: New York Springer US 2020; Springer Nature B.V
• Subjects: Benzoic acid; Biochemistry; Bioreactors; Biotechnology; Bubble columns; Cell culture; Cellulase; Cellulase - metabolism; Chemistry; Chemistry and Materials Science; Culture Media; Domestic wastewater; Enzyme Stability; Fermentation; Formulations; Fungi; Hydrogen-Ion Concentration; Industrial applications; Kinetics; Maltodextrin; Powder; Powders; Reactors; Sorbitol; Stability; Temperature; Trichoderma - enzymology; Trichoderma - metabolism; Trichoderma harzianum; Ultrafiltration; Waste Water - chemistry; Wastewater; FPase; β-Glucosidases; Domestic wastewater; Enzyme formulation; Bubble column bioreactor; Trichoderma harzianum
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-019-03075-1

Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column reactor (6.2 U
FP
/mL, 64.6 U/L h) using the strain
Trichoderma harzianum
TRIC03-LPBII. Cellulases presented optimum pH and temperature between 4 and 5 and 50 and 70 °C, respectively. Enzymatic extract was concentrated through ultrafiltration and then a cellulolytic formulation was prepared with the addition of sorbitol (50% w/v) and benzoic acid (0.05% w/v). High cellulase stability of around 100% was reached after 30 days at 4 °C. The concentrated extract was also dried in a spray-dryer with the addition of maltodextrin at 20% (w/v), resulting in powder enzymatic formulation with 85% stability after 60 days. With these characteristics, the liquid and powder cellulase products have potential to be used in different industrial applications.