Thermostable exo-inulinase production by semicontinuous cultivation of membrane-immobilized Bacillus sp. 11 cells

• Published in: Process biochemistry (1991) Vol. 37; no. 8; pp. 863 - 868
• Main Authors: Uzunova, K., Vassileva, A., Ivanova, V., Spasova, D., Tonkova, A.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-03-2002
• Subjects: Cell immobilization; High fructose syrups; Inulin; Polymer membrane; Thermophilic bacilli; Thermostable exo-inulinase; Polymer membrane; Thermophilic bacilli; High fructose syrups; Thermostable exo-inulinase; Cell immobilization; Inulin
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/S0032-9592(01)00283-7

Growing cells of thermophilic
Bacillus sp. 11, producer of a thermostable exo-inulinase, were immobilized on formaldehyde-activated polysulphone membranes. The biocatalysts obtained showed 1.5–2 fold higher enzyme yields (inulinase and invertase activities) than those of free cells. Active cell growth and no alterations of the membrane structure were observed by scanning electron microscopy (SEM) after 10 days (five cycles) repeated batch cultivation. The residual enzyme activities of the biocatalysts were 60–90% of their initial activities at the end of fifth run of semicontinuous cultivation.