Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides

• Published in: Veterinary research communications Vol. 47; no. 4; pp. 2229 - 2233
• Main Authors: Manna, Camelia, Das, Kinsuk, Mandal, Durgadas, Banerjee, Dipak, Mukherjee, Joydip, Ganguly, Indrajit, Naskar, Shymal, Bag, Sadhan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2023; Springer Nature B.V
• Subjects: Antimicrobial activity; Antimicrobial peptides; Biomedical and Life Sciences; Brief Report; Cell culture; Chemokines; Gene expression; Hepcidin; Innate immunity; Life Sciences; Lipocalin; Mesenchymal stem cells; Peptides; Proteinase inhibitors; RNA-directed DNA polymerase; Stem cells; Surface markers; Therapeutic applications; Umbilical cord; Veterinary Medicine/Veterinary Science; Zoology; Canine mesenchymal stem cells; culture; Antimicrobial peptides; mRNA expression; In vitro culture
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-023-10098-x

Antimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive rate. They represent the first line of active defence systems against invading microorganisms, helping in innate immunity. Besides their therapeutic applications, great attention has also been given to the mesenchymal stem cells (MSCs) due to their antimicrobial activities. The study aimed to observe the mRNA expression profile of few antimicrobial peptides (AMPs) in canine MSCs during standard
in vitro
culture. MSCs were isolated from canine umbilical cord tissue, propagated and characterized by morphology, surface markers and tri-lineage differentiation capability. The mRNA expression of eleven commonly known antimicrobial peptides was checked by Reverse Transcriptase PCR. It has been found for the first time that canine MSCs naturally express the mRNAs of AMPs like C-X-C motif chemokine ligand 8
(CXCL8)
, Elafin
(PI3)
, Hepcidin
(HAMP)
, Lipocalin 2
(LCN2)
and Secretory leukocyte protease inhibitor
(SLPI).
However, their expressions at protein level and, relation with antimicrobial effect of canine MSCs need to be explored.