Apolipoprotein B-100 kinetics and static plasma indices of triglyceride-rich lipoprotein metabolism in overweight men

• Published in: Clinical biochemistry Vol. 38; no. 9; pp. 806 - 812
• Main Authors: Chan, Dick C., Watts, Gerald F., Ng, Theodore W.K., Uchida, Yoshiaki, Sakai, Naohiko, Yamashita, Shizuya, Barrett, P. Hugh R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-09-2005
• Subjects: apoB-100 metabolism; Apolipoprotein B-100; Apolipoprotein B-48; Apolipoproteins B - blood; Apolipoproteins B - metabolism; Cardiovascular disease; Cholesterol; Humans; Lipoproteins - blood; Lipoproteins - metabolism; Male; Obesity; Obesity - blood; Obesity - metabolism; Overweight - physiology; Triglyceride-rich lipoproteins; Triglycerides - blood; Triglycerides - metabolism; apoB-100 metabolism; Obesity; Cardiovascular disease; Triglyceride-rich lipoproteins
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2005.05.009

We examined the association of plasma apolipoprotein (apoB) B-48, remnant-like particle (RLP)-cholesterol and non-HDL cholesterol concentrations with apoB-100 kinetics in overweight-obese men.
Very-low density lipoprotein (VLDL), intermediate-density lipoprotein (IDL) and low-density lipoprotein (LDL) apoB-100 kinetics were measured in 53 men (BMI 33 ± 4 kg/m
2) using stable isotopes and multicompartmental modeling to estimate production rate (PR) and fractional catabolic rate (FCR). Fasting apoB-48 and RLP-cholesterol concentrations were measured using immunoassays. In univariate regression, apoB-48 and RLP-cholesterol were inversely associated with VLDL-apoB-100 FCR and IDL-apoB-100 FCR (
P < 0.01 for all), but not with VLDL-, IDL- and LDL-apoB-100 PRs. Plasma non-HDL-cholesterol concentration was significantly and positively associated with the secretion rate of VLDL-apoB-100 (
P < 0.05), and inversely correlated with the FCR of LDL-apoB-100 (
P < 0.01).
Our findings suggest that in overweight-obese men plasma concentrations of apoB-48, RLP-cholesterol and non-HDL-cholesterol are partly dependent on catabolism of apoB-100 containing lipoproteins.