Antibacterial Activity of Boron Compounds Against Biofilm-Forming Pathogens

• Published in: Biological trace element research Vol. 202; no. 1; pp. 346 - 359
• Main Authors: Celebi, Ozgur, Celebi, Demet, Baser, Sumeyye, Aydın, Elif, Rakıcı, Erva, Uğraş, Serpil, Ağyar Yoldaş, Pınar, Baygutalp, Nurcan Kılıç, Abd El-Aty, A. M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2024; Springer Nature B.V
• Subjects: Ammonium; Ammonium compounds; Ammonium Compounds - pharmacology; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiotics; Bacteria; Biochemistry; Biofilms; Biomedical and Life Sciences; Biotechnology; Blood culture; Borates - pharmacology; Borax; Boron; Boron - pharmacology; Boron compounds; Boron Compounds - pharmacology; Calcium; Cell culture; Cells; Cytotoxicity; Fibroblasts; Fluorine; Fluorine - pharmacology; Life Sciences; Methicillin; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Minimum inhibitory concentration; Nutrition; Oncology; Optical density; Pathogenic bacteria; Pathogens; Plates; Potassium; Sodium; Sodium perborate; Sodium tetraborate; Suspended particulate matter; Toxicity; Tubes; Zinc; Zinc borate; Biofilm; Cell culture; Antibacterial activity; Boron derivatives; Minimum inhibitory concentration
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-023-03768-z

This study aimed to evaluate the antibacterial activity of nine boron derivatives against biofilm-forming pathogenic bacteria. The effect of boron derivatives (CMB, calcium metaborate; SMTB, sodium metaborate tetrahydrate; ZB, zinc borate; STFB, sodium tetra fluorine borate; STB, sodium tetraborate; PTFB, potassium tetra fluor borate; APTB, ammonium pentabo-rate tetrahydrate; SPM, sodium perborate monohydrate; Borax, ATFB, ammonium tetra fluorine borate) on bacteria isolated from blood culture was determined by the minimum inhibitory concentration (MIC) method. Then, biofilm formation potentials on microplates, tubes, and Congo red agar were examined. The cytotoxicity of boron derivatives was determined by using WST-1-based methods. The interaction between the biofilm-forming bacteria, fibroblast cells, and boron derivatives was determined with the infection model. We found that the sodium metaborate tetrahydrate molecule was effective against all pathogens. According to the optical density values detected at 630 nm in microplates, meticillin-resistant
Staphylococcus aureus
was observed to have the most substantial biofilm ability at 0.257 nm. As a result of cytotoxicity studies, it has been determined that a 1 µg/L concentration of boron derivatives is not toxic to fibroblast L929 cells. In cell culture experiments, these boron derivatives have very serious inhibitory activity against biofilm-forming pathogens in a short treatment period, such as 2–4 h. Furthermore, using these molecules on inanimate surfaces affected by biofilms would be appropriate instead of living cells.