The propolis and boric acid can be highly suitable, alone/or as a combinatory approach on ovary ischemia-reperfusion injury

• Published in: Archives of gynecology and obstetrics Vol. 300; no. 5; pp. 1405 - 1412
• Main Authors: Geyikoglu, Fatime, Koc, Kubra, Erol, Huseyin Serkan, Colak, Suat, Ayer, Hafize, Jama, Shukri, Eser, Gizem, Dortbudak, Muhammed Bahaeddin, Saglam, Yavuz Selim
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2019; Springer Nature B.V
• Subjects: Animals; Anti-Infective Agents - pharmacology; Anti-Infective Agents - therapeutic use; Boric Acids - pharmacology; Boric Acids - therapeutic use; Endocrinology; Female; Gynecologic Endocrinology and Reproductive Medicine; Gynecology; Human Genetics; Injuries; Ischemia; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Ovary - drug effects; Ovary - pathology; Oxidative stress; Propolis - pharmacology; Propolis - therapeutic use; Rats; Rats, Sprague-Dawley; Reperfusion Injury - drug therapy; Reperfusion Injury - pathology; Rodents; Tumor necrosis factor-TNF; Ischemia-reperfusion; Ovary; Boric acid; Inflammation; Propolis; Oxidative stress/antioxidant activity
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-019-05303-9

Purpose
Ovarian ischemia-reperfusion (IR) damage continues to be a serious infertility problem. The oxidative stress plays central role in the development of IR injuries. Activation of antioxidants decreases IR injuries; however, the efficacy of antioxidant agents remains controversial. Unfortunately, there has been no evidence for medicinal use of boric acid (BA) and propolis (Prop) on ovarian IR injury on rats so far. This study will provide to reveal the potential applications of the Prop and BA in ovarian IR therapy.
Methods
The Sprague–Dawley rats were randomized into five groups: I—control, II—IR, 3 h of ischemia and 3 h of reperfusion, III and IV—a signal dose of oral BA (7 mg/kg) and Prop (100 mg/kg) alone 1 h before induction of IR, V—Prop and BA together 1 h before induction of IR. SOD (superoxide dismutase), CAT (catalase), GSH (glutathione), MPO (myeloperoxidase), MDA (malondialdehyde), and IL-6 (interleukin-6) levels were quantified by ELISA and the TNF-α (tumor necrosis factor-α), 8-OHdG (8-hydroxylo-2′-deoxyguanosin) and Caspase-3 expressions were performed by immunohistochemical analyses.
Results
BA and Prop pretreatment significantly reduced MPO, MDA, and IL-6 levels and pathologic score in IR rats, with no effects in control group. These agents used in therapy also decreased TNF-α, 8-OHdG and Caspase-3 protein expressions increased by IR. Furthermore, BA and Prop combination showed significant ameliorative effects on ovary injury caused by IR through acting as an antioxidant, anti-inflammatory and antiapoptotic agent.
Conclusion
BA and Prop alone and especially in combination could be developed as therapeutic agents against ovary IR injury.