Effect of tebuconazole and trifloxystrobin on Ceratocystis fimbriata to control black rot of sweet potato: processes of reactive oxygen species generation and antioxidant defense responses

• Published in: World journal of microbiology & biotechnology Vol. 37; no. 9; p. 148
• Main Authors: Mohsin, Sayed Mohammad, Hasanuzzaman, Mirza, Parvin, Khursheda, Morokuma, Masahiro, Fujita, Masayuki
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-09-2021; Springer Nature B.V
• Subjects: Acetates - pharmacology; Antioxidants; Antioxidants - pharmacology; Applied Microbiology; Ascorbic acid; Biochemistry; Biomedical and Life Sciences; Biotechnology; Black rot; Ceratocystis - drug effects; Ceratocystis - growth & development; Ceratocystis fimbriata; Disease control; Disease Resistance; Disks; Drug Synergism; Electrolyte leakage; Environmental Engineering/Biotechnology; Enzymatic activity; Fungicides; Glutathione; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Imines - pharmacology; Ipomoea batatas; Ipomoea batatas - growth & development; Ipomoea batatas - microbiology; Life Sciences; Lipoxygenase; Lipoxygenase - metabolism; Liquid oxygen; Malondialdehyde; Malondialdehyde - metabolism; Microbial Viability - drug effects; Microbiology; Original Paper; Potatoes; Reactive oxygen species; Reactive Oxygen Species - metabolism; Strobilurins - pharmacology; Sweet potatoes; Tebuconazole; Triazoles - pharmacology; Vegetables; Reactive oxygen species; Fungicides; Sweet potato; Antioxidant defense; Ceratocystis fimbriata
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-021-03111-5

Black rot, caused by
Ceratocystis fimbriata
, is one of the most destructive disease of sweet potato worldwide, resulting in significant yield losses. However, a proper management system can increase resistance to this disease. Therefore, this study investigated the potential of using tebuconazole (TEB) and trifloxystrobin (TRI) to improve the antioxidant defense systems in sweet potato as well as the inhibitory effects on the growth of and antioxidant activity in
C. fimbriata
. Four days after inoculating cut surfaces of sweet potato disks with
C. fimbriata
, disease development was reduced by different concentrations of TEB + TRI. Infection by
C. fimbriata
increased the levels of hydrogen peroxide (H
2
O
2
), malondialdehyde (MDA), and electrolyte leakage (EL), and the activity of lipoxygenase (LOX) by 138, 152, 73, and 282%, respectively, in sweet potato disks, relative to control. In the sweet potato disks,
C. fimbriata
reduced the antioxidant enzyme activities as well as the contents of ascorbate (AsA) and reduced glutathione (GSH) by 82 and 91%, respectively, compared with control. However, TEB + TRI reduced the oxidative damage in the
C. fimbriata
-inoculated sweet potato disks by enhancing the antioxidant defense systems. On the other hand, applying TEB + TRI increased the levels of H
2
O
2
, MDA, and EL, and increased the activity of LOX in
C. fimbriata
, in which the contents of AsA and GSH decreased, and therefore, inhibited the growth of
C. fimbriata
. These results suggest that TEB + TRI can significantly control black rot disease in sweet potato by inhibiting the growth of
C. fimbriata
.