The Role of β-Adrenergic Receptors in the Regulation of the Functions of Innate Immune Cells during Cold Stress In Vivo

• Published in: Bulletin of experimental biology and medicine Vol. 173; no. 1; pp. 72 - 76
• Main Authors: Gein, S. V., Karnaukhova, A. V.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-05-2022; Springer Nature B.V
• Subjects: Adrenergic receptors; Biomedical and Life Sciences; Biomedicine; Catecholamines; Cell Biology; Cold; Cytokines; Experiments; Genetics; IL-1β; Immune system; Interleukin 10; Internal Medicine; Laboratory Medicine; Macrophages; Microbicides; Pathology; Peritoneum; Phagocytes; Propranolol; macrophage; cold stress; cytokines; adrenoreceptors
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05496-1

It was found that 10-min cold stress enhanced stimulated production of ROS, while 60-min cold stress increased both spontaneous and stimulated ROS production by peritoneal macrophages. β-Adrenergic receptor blockade leveled the effect of 10-min stress in stimulated cultures and the effect of 60-min stress in spontaneous cultures. None variants of cold stress affected spontaneous and stimulated production of IL-1β. We observed an increase in the production of IL-1β in stimulated cultures from animals subjected to 10- and 60-min stress against the background of propranolol. At the same time, both variants of cold exposure, irrespective of β-adrenergic receptor blockade, stimulated IL-10 synthesis in spontaneous and activated samples. None of the used models of cold exposure affected the phagocytic activity of peritoneal macrophages. Thus, β-adrenergic receptors are directly involved in the regulation of cytokine production and microbicidal potential of macrophages in acute cold stress.