TLR4 and TLR9 polymorphisms are not associated with either rheumatoid arthritis or systemic lupus erythematosus in Mexican patients

• Published in: Molecular biology reports Vol. 48; no. 4; pp. 3561 - 3565
• Main Authors: Aranda-Uribe, Ivan Sammir, López-Vázquez, Juan Carlos, Barbosa-Cobos, Rosa Elda, Ramírez-Bello, Julian
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-04-2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Genetic analysis; Haplotypes; Histology; Inflammation; Life Sciences; Lupus; Microenvironments; Morphology; Nuclease; Original Article; Rheumatoid arthritis; Risk factors; Single-nucleotide polymorphism; Systemic lupus erythematosus; TLR4 protein; TLR9 protein; Toll-like receptors; Systemic lupus erythematosus; Single nucleotide variants; Susceptibility; Rheumatoid arthritis; TLR9; TLR4
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06371-4

Toll-like receptor (TLR)-mediated signaling pathways induce a proinflammatory microenvironment to eradicate pathogens. However, in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), TLRs can promote chronic inflammation. It has been shown that some
TLR4
and
TLR9
single nucleotide polymorphisms (SNPs) are risk factors for RA and SLE, but these findings have not been replicated in all populations; thus, results are inconclusive. We evaluated the
TLR4
Asp299Gly, Thr399Ile, − 1892G/A SNPs, and the
TLR9
Pro545Pro SNP to assess potential associations with RA and SLE in Mexican patients. This study included 474 patients with RA, 283 patients with SLE, and 424 healthy controls. We used a 5′ nuclease allelic discrimination assay to genotype individuals for the four
TLR4
and
TLR9
polymorphisms. We found that the genotype or allelic frequencies of the
TLR4
Asp299Gly, Thr399Ile, − 1892G/A, and
TLR9
Pro545Pro polymorphisms were similar between patients and controls. We found no association under different genetic models. A haplotype analysis of
TLR4
showed no association with either RA or SLE. We found no significant differences in the allelic or genotypic frequencies of
TLR4
Asp299Gly, Thr399IIe, − 1892G/A, or
TLR9
Pro545Pro between patients and controls. These findings suggested that these variants are not risk factors for RA or SLE in Mexican patients.