Effects of probiotics on pentylenetetrazol-induced convulsions in mice

• Published in: Epilepsy research Vol. 176; p. 106723
• Main Authors: Sabouri, Salehe, Kangi, Sahar, Najimi, Sara, Rahimi, Hamid-Reza
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2021
• Subjects: Animals; Anticonvulsants - therapeutic use; Bifidobacterium; Diazepam - pharmacology; Diazepam - therapeutic use; Epilepsy; Lactobacillus; Mice; Pentylenetetrazole - toxicity; Probiotics - pharmacology; Probiotics - therapeutic use; Seizures; Seizures - drug therapy; Tonic-Clonic; PB; Bifidobacterium; FLZ; NS; Epilepsy; PTZ; Tonic-Clonic; DZP; Seizures; Lactobacillus
• ISSN: 0920-1211; 1872-6844
• DOI: 10.1016/j.eplepsyres.2021.106723

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•Probiotics have some influences on the central nervous system.•Probiotics enhance the anticonvulsant effect of diazepam.•GABAergic system may be involved in anticonvulsant effect of probiotics.
There are some reports of the effect of the gut microbiota on the central nervous system. The aim of this study was to find out the effect of probiotics on pentylenetetrazol (PTZ)-induced convulsions in mice.
The mice were pretreated with probiotic powder (Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum) suspended in normal saline by intragastric gavage (IG-gavage) for 14 (group 1) or 28 (group 2) days prior to injection of PTZ (90 mg/kg, intraperitoneally). Diazepam (DZP, 1 mg/kg, intraperitoneally) was used as the reference drug. The latency and duration of induced convulsion, as well as mortality protection percentage were recorded 30 min after PTZ injection. For the next step, flumazenil (FLZ) was used to block the effect of DZP.
Pretreatment with probiotics for 14 or 28 days had not a significant effect on the latency and duration of seizures induced by PTZ. Neither seizure nor mortality was observed in co-administration of probiotics with DZP. FLZ pretreatment decreased the DZP-induced seizure latency; however, FLZ could not have such an effect in probiotic and DZP group.
Probiotics alone did not show anticonvulsant effects, but enhanced the anticonvulsant effect of DZP; this suggests the involvement of GABAergic system.