Use of whole blood for analysis of disease-associated biomarkers
Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of plasma or serum. We present a feasibility study de...
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| Published in: | Analytical biochemistry Vol. 437; no. 1; pp. 59 - 61 |
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| Main Authors: | , , , , , |
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| Language: | English |
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01-06-2013
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| Abstract | Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of plasma or serum. We present a feasibility study demonstrating potential use of fresh or frozen whole blood to detect soluble biomarkers using an enzyme-linked immunosorbent assay (ELISA)-based method. Good correlation between levels of soluble CD25 in plasma and whole blood of healthy individuals or Alzheimer’s patients was established. These results provide a basis for development of a novel biosensor approach for disease-associated biomarker detection in whole blood. |
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| AbstractList | Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of plasma or serum. We present a feasibility study demonstrating potential use of fresh or frozen whole blood to detect soluble biomarkers using an enzyme-linked immunosorbent assay (ELISA)-based method. Good correlation between levels of soluble CD25 in plasma and whole blood of healthy individuals or Alzheimer’s patients was established. These results provide a basis for development of a novel biosensor approach for disease-associated biomarker detection in whole blood. |
| Author | Hart, John P. Pemberton, Roy M. Wilcock, Gordon May, Jennifer E. Doran, Olena McLeod, Julie |
| Author_xml | – sequence: 1 givenname: Jennifer E. surname: May fullname: May, Jennifer E. email: jennifer2.may@uwe.ac.uk organization: Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK – sequence: 2 givenname: Roy M. surname: Pemberton fullname: Pemberton, Roy M. organization: Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK – sequence: 3 givenname: John P. surname: Hart fullname: Hart, John P. organization: Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK – sequence: 4 givenname: Julie surname: McLeod fullname: McLeod, Julie organization: Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK – sequence: 5 givenname: Gordon surname: Wilcock fullname: Wilcock, Gordon organization: Optima Project, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK – sequence: 6 givenname: Olena surname: Doran fullname: Doran, Olena organization: Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23467100$$D View this record in MEDLINE/PubMed |
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| Keywords | Whole blood Biomarkers Plasma CD25 ELISA |
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| SubjectTerms | Adult Alzheimer Disease - blood Biomarkers Biomarkers - blood Biosensing Techniques biosensors Blood Blood Chemical Analysis - methods blood serum CD25 Cryopreservation ELISA enzyme-linked immunosorbent assay hematologic tests Humans Interleukin-2 Receptor alpha Subunit - blood Middle Aged monitoring patients Plasma Whole blood |
| Title | Use of whole blood for analysis of disease-associated biomarkers |
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