Assessment of tissue distribution and concentration of β-cryptoxanthin in response to varying amounts of dietary β-cryptoxanthin in the Mongolian gerbil

• Published in: British journal of nutrition Vol. 111; no. 6; pp. 968 - 978
• Main Authors: LA FRANO, Michael R, CHENGHAO ZHU, BURRI, Betty J
• Format: Journal Article
• Language: English
• Published: Cambridge Cambridge University Press 28-03-2014
• Subjects: Abdominal Fat - chemistry; Animals; Biological and medical sciences; Carotenoids - analysis; Citrus; Cryptoxanthins; Diet; Feeding. Feeding behavior; Female; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; Gerbillinae - metabolism; Humans; Intestines - chemistry; Liver - chemistry; Models, Animal; Tissue Distribution - drug effects; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitamin A - analysis; Weight Gain - drug effects; Xanthophylls - administration & dosage; Xanthophylls - analysis; Xanthophylls - pharmacokinetics; Nutrition; β-Cryptoxanthin; Cryptoxanthin; Rodentia; Vitamin; Gerbils; Retinol; Micronutrient; Tissue; Vitamin A; Vertebrata; Mammalia; Carotenoids; Animal; Tissue distribution; Carotenoid; Gerbil; Concentration distribution
• ISSN: 0007-1145; 1475-2662
• DOI: 10.1017/S0007114513003371

There is a general lack of knowledge regarding the absorption and tissue storage of the provitamin A carotenoid β-cryptoxanthin. The present study investigated the whole-body tissue distribution of β-cryptoxanthin in an appropriate small animal model, the Mongolian gerbil (Meriones unguiculatus), for human provitamin A carotenoid metabolism. After 5 d of carotenoid depletion, five gerbils were euthanised for baseline measurements. The remaining gerbils were placed in three weight-matched treatment groups (n 8). All the groups received 20 μg/d of β-cryptoxanthin from tangerine concentrate, while the second and third groups received an additional 20 and 40 μg/d of pure β-cryptoxanthin (CX40 and CX60), respectively, for 21 d. During the last 2 d of the study, urine and faecal samples of two gerbils from each treatment group were collected. β-Cryptoxanthin was detected in the whole blood, and in twelve of the fourteen tissues analysed. Most tissues resembled the liver, in which the concentrations of β-cryptoxanthin were significantly higher in the CX60 (17·8 (sem 0·7) μg/organ; P= 0·004) and CX40 (16·2 (sem 0·9) μg/organ; P= 0·006) groups than in the CX20 group (13·3 (sem 0·4) μg/organ). However, in intestinal tissues, the concentrations of β-cryptoxanthin increased only in the CX60 group. Despite elevated vitamin A concentrations in tissues at baseline due to pre-study diets containing high levels of vitamin A, β-cryptoxanthin maintained those vitamin A stores. These results indicate that β-cryptoxanthin is stored in many tissues, potentially suggesting that its functions are widespread.