EVALUATION OF GENETIC STABILITY IN CRYOPRESERVED PRUNUS

• Published in: In vitro cellular & developmental biology. Plant Vol. 38; no. 5; pp. 493 - 500
• Main Authors: HELLIOT, B., MADUR, D., DIRLEWANGER, E., DE BOUCAUD, M. T.
• Format: Journal Article
• Language: English
• Published: Wallingford CABI Publishing 01-09-2002; Cambridge University Press; Springer Nature B.V; Springer
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Cryopreservation; Freezing; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetic stability; Genetics and breeding of economic plants; Life Sciences; Orchards; Plant cells; Plant material; Plantlets; Plants; Ploidies; Rootstocks; Seed trees; Vegetal Biology; Rosaceae; Cytology; Random amplified polymorphic DNA marker; Regeneration; Phenotype; Cryopreservation; Dicotyledones; Angiospermae; Prunus; Plantlet; Spermatophyta; Apex; Amplified fragment length polymorphism marker; RAPD; GENETIQUE
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1079/IVP2002325

The evaluation of the genetic stability of Prunus Ferlenain plants regenerated from cryopreserved apices was investigated. The analysis of plants recovered from frozen material was performed at the phenotypic (developmental competence), cytological (chromosomal preparations) and molecular level [random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP)]. No genetic change was detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material, including leaves of the mother tree kept in an orchard and vitroplantlets regenerated from non-frozen apices. This result suggested that the procedure used for Prunus cryopreservation could be used for long-term conservation. The relevance of each strategy for the genetic stability evaluation of the cryopreserved material is discussed.