Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria

• Published in: Applied biochemistry and microbiology Vol. 49; no. 5; pp. 458 - 463
• Main Authors: Purish, L. M, Asaulenko, L. G, Abdulina, D. R, Voychuk, S. I, Iutynskaya, G. A
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01-09-2013; Springer US; Springer Nature B.V
• Subjects: Bacillus subtilis; Bacteria; Binding sites; Biochemistry; biofilm; Biofilms; Biomedical and Life Sciences; carbohydrate composition; Carbohydrates; Corrosion; Desulfovibrio; electron microscopy; Gold; lectins; Life Sciences; Matrix; Medical Microbiology; Microbial corrosion; Microbiology; Polymer films; Pseudomonas aeruginosa; Colloidal Gold; Carbohydrate Component; Colloidal Gold Particle; Apply Biochemistry; Desulfovibrio
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683813050104

The carbohydrate components of biofilms of corrosion-aggressive bacteria were studied by transmisstion electron microscopy using lectins labeled with colloidal gold. N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, and neutral carbohydrates D-glucose and D-mannose were found within the exopolymeric matrix. Lectins with equal carbohydrate specificity demonstrated different degrees of interaction with the carbohydrate components of bacterial biofilms. To identify N-acetyl-D-galactosamine in biofilms of Desulfovibrio sp. 10 and Bacillus subtilis 36, the LBA lectin appeared to be most specific; in the case of N-acetyl-D-glucosamine in biofilms of B. subtilis 36 and Pseudomonas aeruginosa 27, the WGA lectin. During visualization of neutral carbohydrates in the studied cultures, the PSA lectin was most specific. We have shown that lectins labeled with colloidal gold could be used as an express method for the identification and localization of carbohydrates in glycopolymers of the biofilm exopolymeric matrix.