Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate t...
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| Published in: | Brazilian journal of microbiology Vol. 43; no. 3; pp. 1005 - 1009 |
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| Format: | Journal Article |
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São Paulo
Springer Nature B.V
01-07-2012
Sociedade Brasileira de Microbiologia |
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| Abstract | Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. [PUBLICATION ABSTRACT] |
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| AbstractList | Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. [PUBLICATION ABSTRACT] Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. |
| Author | Esteves, P.A. Bianchi, P Pérez, R Franco, G Furtado, A Hübner, S.O. Eliopulos, N Sosa, K Puentes, R |
| AuthorAffiliation | Universidad de la República Embrapa Universidade Federal de Pelotas |
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| Copyright | Copyright Sociedade Brasileira de Microbiologia 2012 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
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| DOI | 10.1590/S1517-83822012000300022 |
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| Keywords | South America Isolation Molecular characterization Canine Parvovirus 2c |
| Language | English |
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| References_xml | – volume: 70 start-page: 769 issue: 8 year: 2008 end-page: 775 article-title: Chronological Analysis of Canine Parvovirus Type 2 Isolates in Japan publication-title: J. Vet. Med. Sci contributor: fullname: Ohshima, T.; Mochizuki, M.; Kawakami, K.; Kishi, M.; Tohya, Y.; Mochizuki, M. – volume: 17 start-page: 133 year: 2005 end-page: 138 article-title: Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant publication-title: J Vet Diagn Invest contributor: fullname: Decaro, N.; Desario, C.; Campolo, M.; Elia, G.; Martella, V.; Ricci, D.; Lorusso, E.; Buonavoglia, C. – volume: 159 start-page: 141 year: 2009 end-page: 145 article-title: Molecular characterization of canine parvovirus strains in Argentina: Detection of the pathogenic variant CPV2c in vaccinated dogs publication-title: J. Virol. Methods contributor: fullname: Calderon, M.; Mattion, N.; Bucafusco, D.; Fogel, F.; Remorini, P.; La Torre, J. – volume: 124 start-page: 147 year: 2007 end-page: 152 article-title: First detection of canine parvovirus type 2c in South America publication-title: Vet. Microbiol contributor: fullname: Pérez, R.; Francia, L.; Romero, V.; Maya, L.; López, I.; Hernández, M. – volume: 70 start-page: 1305 issue: 12 end-page: 1314 article-title: Between vaccine and Field Strains of Canine Parvovirus is Revealed by Isolation of Virus in Canine and Feline Cell Cultures publication-title: J. Vet. Med. Sci – volume: 41 issue: 39 year: 2010 article-title: The emergence of parvoviruses of carnivores publication-title: Vet. Res contributor: fullname: Hoelzer, K.; Parrish, C. – volume: 40 start-page: 465 issue: 3 year: 2009 end-page: 469 article-title: First detection of canine parvovirus type 2c in Brazil publication-title: Braz. J. Microbiol contributor: fullname: Streck, A.; Kunzler, C.; Rathje, K.; Zang, L.; Dubina, L.; Wageck, C. – volume: 52 start-page: 316 year: 2005 end-page: 319 article-title: New approaches for the molecular characterization of canine parvovirus type 2 strains publication-title: J. Vet. Med. B Infect Dis. Vet. Public Health contributor: fullname: Decaro, N.; Elia, G.; Campolo, M.; Desario, C.; Lucente, M.; Bellacicco, A.; Buonavoglia, C. – volume: 117 start-page: 9 year: 2006 end-page: 13 article-title: Evolution of canine parvovirus: a need for new vaccines? Vet publication-title: Microbiol contributor: fullname: Truyen, U. – year: 2008 publication-title: Recombination contributor: fullname: Mochizuki, M.; Ohshima, T.; Une, Y.; Yachi, A. – volume: 12 start-page: 1 year: 1986 end-page: 6 article-title: An improved hemagglutination test for study of canine parvovirus publication-title: Vet. Microbiol contributor: fullname: Senda, M.; Hirayama, N.; Yamamoto, H.; Kurata, K. – volume: 82 start-page: 3021 year: 2001 end-page: 3025 article-title: Evidence for evolution of canine parvovirus type 2 in Italy publication-title: J. Gen. Virol contributor: fullname: Buonavoglia, C.; Martella, V.; Pratelli, A.; Tempesta, M.; Cavalli, A.; Buonavoglia, D.; Bozzo, G.; Elia, G.; Decaro, N.; Carmichael, L. |
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| Title | Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies |
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