Immunological studies of poisonous anacardiaceae: effect of vehicle on absorption of 3-n-pentadecylcatechol and its diacetate ester derivative after oral feeding in rats

• Published in: Journal of pharmaceutical sciences Vol. 70; no. 7; p. 829
• Main Authors: Skierkowski, P, ElSohly, M A, Harland, E C, King, B S, Murphy, J C, Watson, E S
• Format: Journal Article
• Language: English
• Published: United States 01-07-1981
• Subjects: Administration, Oral; Animals; Bile - metabolism; Catechols - administration & dosage; Catechols - metabolism; Ethanol; Feces - analysis; Female; Intestinal Absorption; Oils; Pharmaceutical Vehicles; Rats
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600700738

Tritium-labeled 3-n-pentadecylcatechol and its diacetate ester were fed to Sprague-Dawley rats. Both compounds were dissolved in ethanol and in corn oil vehicles and administered by gavage. The rats were placed in metabolic cages, and the urine and feces was determined by liquid scintillation counting, and the percentage of the administered dose was utilized as a measure of absorption. While there was no difference between the absorption of either compound, absorption was affected by the vehicle. Approximately 30% of the administered radioactivity appeared in the urine when ethanol was the vehicle, but about half that amount (14%) was excreted in the urine when the compounds were dissolved in corn oil. A subsequent bile cannulation study showed that the balance of the radioactivity found in the feces was not a result of biliary excretion. The majority of the activity recovered from urine and feces was eliminated within 48 hr after dosing. These data indicate that oil is a poor vehicle for GI absorption of urushiol components.