effect of cations on the hydrolysis of lactose and the transferase reactions catalysed the beta-galactosidase from six strains of lactic acid bacteria

• Published in: Applied microbiology and biotechnology Vol. 46; no. 1; pp. 22 - 27
• Main Authors: Garman, J, Coolbear, T, Smart, J
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-08-1996
• Subjects: beta-Galactosidase - metabolism; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Cations - pharmacology; dairy products; Disaccharides - metabolism; food microbiology; food processing; Fundamental and applied biological sciences. Psychology; Gram-Positive Bacteria - enzymology; Hydrolysis; Kinetics; Lactic Acid; Lactose - metabolism; Mission oriented research; Physiology and metabolism; Sodium Ions; Lactic acid bacteria; Lactobacillus casei; Environmental factor; Potassium Ions; Actinomycetales; Transglycosylation; Enzymatic activity; Substrate specificity; Magnesium Ions; Bacteria; Lactobacillaceae; Micrococcales; β-Galactosidase; Actinomycetaceae; Enzyme; Hydrolysis; Streptococcaceae; Lactobacillus delbrueckii subsp. bulgaricus; Lactose; Lactococcus lactis subsp. lactis; Streptococcus salivarius subsp. thermophilus; Glycosidases; Dairy industry; Hydrolases; Actinomycetes; Cations; Kinetic parameter; O-Glycosidases; Application; Bifidobacterium bifidum; Comparative study
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530050778

Beta-Galactosidases from Lactobacillus delbruekii subsp. bulgaricus 20056, Lb. cased 20094, Lactococcus lactis subsp. lactis 7962, Streptococcus thermophilus TS2, Pediococcus pentosaceus PE39 and Bifidobacterium bifidum 1901 were partially purified. The rate of hydrolysis of lactose given by the predominant beta-galactosidase activity from each of the bacteria studied was in all cases enhanced by Mg2+, while the effect of K+ and Na+ differed from strain to strain. The beta-galactosidases from all strains also catalysed transgalactosylation reactions. The types of oligosaccharides produced appeared to be very similar in each case, but the rates of their production differed. All the beta-galactosidases were also capable of hydrolysing galactosyl-lactose although, unlike the other bacteria studied, Lb. delbruekii subsp. bulgaricus 20056 and Lc. lactis subsp. lactis 7962 were unable to utilise galactosyl-lactose as a carbon source for growth.