Hippocampal Acetylation may Improve Prenatal-Stress-Induced Depression-Like Behavior of Male Offspring Rats Through Regulating AMPARs Expression

• Published in: Neurochemical research Vol. 42; no. 12; pp. 3456 - 3464
• Main Authors: Lu, Yong, Zhang, Junli, Zhang, Lin, Dang, Shaokang, Su, Qian, Zhang, Huiping, Lin, Tianwei, Zhang, Xiaoxiao, Zhang, Yurong, Sun, Hongli, Zhu, Zhongliang, Li, Hui
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2017; Springer Nature B.V
• Subjects: Acetylation; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Hippocampus; Mental depression; Neurochemistry; Neurology; Neurosciences; Offspring; Original Paper; Prenatal experience; Rats; Rodents; Sucrose; Sugar; Swimming; Trichostatin A; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; Depression-like behavior; Offspring; AMPARs; Acetylation; Prenatal stress
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-017-2393-7

This study is to determine the role and mechanism of hippocampal acetylation in prenatal stress (PS) induced depression-like behavior of male offspring rats. PS-induced depression rat model was established. Sucrose preference and forced swim test were used to observe the behavior changes of male offspring rats. Hippocampal acetylation was induced by Trichostatin A injection. Quantitative real-time PCR and Western blot were used to determine the changes of AMPARs in acetylated hippocampus. The behavioral tests proved that AMPA was involved in the PS-induced depression-like behavior in offspring rats. Hippocampal acetylation significantly increased the preference to sucrose of PS-induced offspring rats and reduced the immobile time in forced swimming test, suggesting that acetylation could improve PS-induced depression-like behaviors. In addition, PS inhibited the expression levels of GluA1-3 subunits of AMPARs in the offspring hippocampus, while Hippocampal acetylation could reverse this effect by increasing GluA1-3 expression. PS-induced reduction of GluA1-3 subunits of AMPARs may be an important potential mechanism of offspring depression. Hippocampal acetylation may improve PS-induced offspring depression-like behavior through the enhanced expression of AMPARs (GluA1-3 subunits).