Soluble laminin and arginine-glycine-aspartic acid containing peptides differentially regulate type IV collagenase messenger RNA, activation, and localization in testicular cell culture

• Published in: Biology of reproduction Vol. 45; no. 3; pp. 387 - 394
• Main Authors: Q X Sang, E W Thompson, D Grant, W G Stetler-Stevenson, S W Byers
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 01-09-1991
• Subjects: Amino Acid Sequence; Animals; Blotting, Western; Cell Membrane - enzymology; Enzyme Activation; Gelatinases; Gene Expression Regulation - drug effects; Laminin - pharmacology; Male; Microbial Collagenase - genetics; Microbial Collagenase - metabolism; Molecular Sequence Data; Pepsin A - metabolism; Peptide Fragments - chemistry; Peptide Fragments - pharmacology; Rats; Rats, Inbred Strains; RNA, Messenger - analysis; RNA, Messenger - metabolism; Testis - chemistry; Testis - metabolism; Tissue Distribution
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod45.3.387

Rat testicular cells in culture produce several metalloproteinases including type IV collagenases (Sang et al. Biol Reprod
1990; 43:946-955, 956-964). We have now investigated the regulation of testicular cell type IV collagenase and other metalloproteinases
in vitro. Soluble laminin stimulated Sertoli cell type IV collagenase mRNA levels. However, three peptides corresponding to
different domains of the laminin molecule (CSRAKQAASIKVASADR, FALRGDNP, CLQDGDVRV) did not influence type IV collagenase mRNA
levels. Zymographic analysis of medium collected from these cultures revealed that neither soluble laminin nor any of the
peptides influenced 72-kDa type IV collagenase protein levels. However, peptide FALRGDNP resulted in both, a selective increase
in two higher molecular-weight metalloproteinases (83 kDa and 110 kDa and in an activation of the 72-kDa rat type IV collagenase.
Interleukin-1, phorbol ester, testosterone, and FSH did not affect collagenase activation. Immunocytochemical studies demonstrated
that the addition of soluble laminin resulted in a redistribution of type IV collagenase from intracellular vesicles to the
cell-substrate region beneath the cells. Peptide FALRGDNP induced a change from a vesicular to peripheral plasma membrane
type of staining pattern. Zymography of plasma membrane preparations demonstrated triton-soluble gelatinases of 76 kDa, 83
kDa, and 110 kDa and a triton-insoluble gelatinase of 225 kDa. These results indicate that testicular cell type IV collagenase
mRNA levels, enzyme activation, and distribution are influenced by laminin and RGD-containing peptides.