Immunochip for Syphilis Serodiagnostics with the Use of Extended Array of Treponema pallidum Recombinant Antigens

• Published in: Bulletin of experimental biology and medicine Vol. 165; no. 6; pp. 767 - 771
• Main Authors: Runina, A. V., Katunin, G. L., Filippova, M. A., Zatevalov, A. M., Kubanov, A. A., Deryabin, D. G.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-10-2018; Springer Nature B.V
• Subjects: Algorithms; Antibodies, Bacterial - blood; Antigens; Antigens, Bacterial - immunology; Bacterial infections; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Bacterial Proteins - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Biology; Discriminant analysis; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique, Indirect; Humans; Immunoglobulin G; Immunoglobulin G - blood; Internal Medicine; Laboratory Medicine; Lipoproteins - metabolism; Membrane proteins; Membrane Proteins - immunology; Pathology; Point-of-Care Testing; Protein transport; Recombinant Proteins - immunology; Reproducibility of Results; Sensitivity and Specificity; Syphilis; Syphilis - blood; Syphilis - diagnosis; Syphilis Serodiagnosis - instrumentation; Treponema pallidum; Treponema pallidum - immunology; biochips; indirect immunofluorescense test; syphilis; laboratory diagnostics; Treponema pallidum
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-018-4261-0

An immunochip for multiple parallel detection of specific serum IgG in serological screening for syphilis is based on the use of an extended array of
Treponema pallidum
recombinant proteins and includes traditionally used immunodominant antigens (Tp15, Tp17, Tp47, and TmpA) and new synthetic proteins (Tp0277, Tp0319, Tp0453, Tp0684, Tp0965, and Tp1038). The use of individual antigens has demonstrated high analytical value of Tp0277 (periplasmatic C-terminal protease), Tp0319 (cytoplasmic membrane-associated lipoprotein TmpC), and external membrane-associated protein Tp0453 with transporting function, all of them improving significantly the efficiency of screening for syphilis in comparison with the traditional array of antigens. Multiparametric analysis of the results obtained on the immunochip with the use of linear discriminant analysis confirmed the efficiency of extended array of
T. pallidum
diagnostic antigens. Due to proposed modification, the “positive” and “negative” sera are clearly differentiated: the serological study showed 94.1% sensitivity and 100% specificity.