Cytotoxicity and anticancer activity of natural rubber latex particles for cancer cells

• Published in: Materials today chemistry Vol. 5; pp. 63 - 71
• Main Authors: Furuya, Mitsuru, Shimono, Naoki, Yamazaki, Kazuyuki, Domura, Ryota, Okamoto, Masami
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-09-2017
• Subjects: Apoptosis; Cancer cells; Cell cycle; Cytotoxicity; Natural rubber latex; Cytotoxicity; Natural rubber latex; Cancer cells; Cell cycle; Apoptosis
• ISSN: 2468-5194; 2468-5194
• DOI: 10.1016/j.mtchem.2017.07.001

To broaden the knowledge of cytotoxicity of natural rubber latex (NRL) nanoparticles we for the first time examined the latex biocompatibility in vitro against a panel of cancer cells (A549, A2780, and MDA-MB-231). Owing to fractionation of NRL nanoparticles by ultra-centrifuge, the effect of the non-rubber constituents (intermediate of 5.8 wt% and sediment of 0.2 wt%) on the cytotoxicity was clarified. For intermediate constituent, the half maximal inhibitory concentration (IC50) values at 24 h was 1.05 mg/mL for A549 cells, which was one order of magnitude higher in toxicity as compared to that for A2780 (0.24 mg/mL) and MDA-MB-231 (0.36 mg/mL) cells. In addition, profound studies including cell cycle arrest abilities and apoptosis induction profiles against cancer cells were discussed in detail. It was found that the constituents exhibit some significant effect on the cell cycle arrest and trigger apoptosis for A2780 cells. This effective apoptosis induction profiles was more prominent in MDA-MB-231 cells incubated with NRL nanoparticles and sediment loading conditions. The percentage of apoptotic cells was ca. 6–8% of the total cells.
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•The cytotoxicity of NRL nanoparticles for cancer cells was examined.•The effect of the non-rubber constituents on the cytotoxicity was clarified.•The cell cycle arrest and apoptosis induction profiles were discussed.•The intercellular accumulation of NRL particles near to the nucleus was clarified.