Expression of Bruton’s tyrosine kinase in B-cell neoplasms evaluated by flow cytometry

• Published in: Clinical and experimental medicine Vol. 17; no. 4; pp. 499 - 504
• Main Authors: Marcondes, Natália Aydos, Fernandes, Flavo Beno, Alegretti, Ana Paula, Faulhaber, Gustavo Adolpho Moreira
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-11-2017; Springer Nature B.V
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Female; Flow Cytometry; Hematology; Humans; Internal Medicine; Kinases; Lymphoma, B-Cell - pathology; Male; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Article; Protein-Tyrosine Kinases - analysis; T cell receptors; Tumors; Young Adult; B-cell neoplasm; BTK; Bruton’s tyrosine kinase; Flow cytometry
• ISSN: 1591-8890; 1591-9528
• DOI: 10.1007/s10238-016-0447-9

Bruton’s tyrosine kinase (BTK) is a cytoplasmatic protein that is part of the B-cell antigen receptor signaling pathway. Our aim was to evaluate the expression of BTK in B-cell neoplasms and compare it to normal B-cell lymphocytes. After surface staining with CD19 and CD45, flow cytometry staining for intracellular BTK was performed in leukemic or mature B-cells from bone marrow or peripheral blood samples. No differences in BTK expression were identified between groups, or in comparison to control samples, there was no association between BTK expression and the clinical variables evaluated. BTK expression in B-cell neoplasms was similar to that of normal B-cell lymphocytes.