Protective effect of Uncaria tomentosa extract against oxidative stress and genotoxicity induced by glyphosate-Roundup® using zebrafish (Danio rerio) as a model

• Published in: Environmental science and pollution research international Vol. 25; no. 12; pp. 11703 - 11715
• Main Authors: Santo, Glaucia Dal, Grotto, Alan, Boligon, Aline A., Da Costa, Bárbara, Rambo, Cassiano L., Fantini, Emily A., Sauer, Elisa, Lazzarotto, Luan M. V., Bertoncello, Kanandra T., Júnior, Osmar Tomazelli, Garcia, Solange C., Siebel, Anna M., Rosemberg, Denis B., Magro, Jacir Dal, Conterato, Greicy M. M., Zanatta, Leila
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2018; Springer Nature B.V
• Subjects: Animals; Antioxidants; Antioxidants - pharmacology; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bioactive compounds; Biocompatibility; Brain damage; Cat's Claw - chemistry; Danio rerio; Deoxyribonucleic acid; DNA; DNA Damage; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Exposure; Female; Genotoxicity; Glycine - analogs & derivatives; Glycine - antagonists & inhibitors; Glycine - toxicity; Glyphosate; Herbicides; Herbicides - antagonists & inhibitors; Herbicides - toxicity; Lipid Peroxidation; Liver; Liver - drug effects; Male; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Phenolic compounds; Phenols; Plant Extracts - pharmacology; Plant species; Research Article; South America; Thiols; Toxicity; Uncaria tomentosa; Waste Water Technology; Water Management; Water Pollution Control; Zebrafish; Zebrafish; Bioactive compounds; Antioxidant activity; Pesticides; Acute toxicity
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-018-1350-6

Oxidative stress and DNA damage are involved in the glyphosate-based herbicide toxicity.
Uncaria tomentosa
(UT; Rubiaceae) is a plant species from South America containing bioactive compounds with known beneficial properties. The objective of this work was to evaluate the antioxidant and antigenotoxic potential of UT extract in a model of acute exposure to glyphosate-Roundup® (GR) in zebrafish (
Danio rerio
). We showed that UT (1.0 mg/mL) prevented the decrease of brain total thiols, the increase of lipid peroxidation in both brain and liver, and the decrease of liver GPx activity caused after 96 h of GR (5.0 mg/L) exposure. In addition, UT partially protected against the increase of micronucleus frequency induced by GR exposure in fish brain. Overall, our results indicate that UT protects against damage induced by a glyphosate-based herbicide by providing antioxidant and antigenotoxic effects, which may be related to the phenolic compounds identified in the extract.