Principles for Construction of Bioluminescent Enzyme Biotests for Analysis of Complex Media

• Published in: Doklady. Biochemistry and biophysics Vol. 485; no. 1; pp. 107 - 110
• Main Authors: Kalyabina, V. P., Esimbekova, E. N., Torgashina, I. G., Kopylova, K. V., Kratasyuk, V. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2019; Springer Nature B.V
• Subjects: Bacteria - enzymology; Bacterial Proteins - chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Enzymes; FMN Reductase - chemistry; Food Analysis - methods; Life Sciences; Luciferases - chemistry; Luminescent Measurements - methods; Luminous bacteria; Molecular Biology; NAD; Oxidoreductase
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672919020042

In this study, we formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media, which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables, which is based on using the luminous bacteria enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.