Soil polluted with nano ZnO reveals unstable bacterial communities and decoupling of taxonomic and functional diversities

• Published in: The Science of the total environment Vol. 889; p. 164285
• Main Authors: Dinesh, R., Sreena, C.P., Sheeja, T.E., Kumar, I.P. Vijesh, Praveena, R., Charles, Sona, Srinivasan, V., Jayarajan, K., Sajith, V., Subila, K.P., Haritha, P.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-09-2023
• Subjects: Metabolic quotient; Metal oxide nanoparticles; Predictive metagenomic profiling; Soil enzyme activity; Soil microbial biomass; Soil pollution; Metabolic quotient; Metal oxide nanoparticles; Soil enzyme activity; Soil pollution; Soil microbial biomass; Predictive metagenomic profiling
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.164285

Due to relentless production and disposal of nano zinc oxide (nZnO), it has become critical to comprehend the serious risks large-scale accumulation of nZnO pose to bacterial communities in soil. The primary objective was to evaluate the changes in bacterial community structure and associated functional pathways through predictive metagenomic profiling and subsequent validation through Quantitative Realtime PCR in soil spiked with nZnO (0, 50, 200, 500 and 1000 mg Zn kg−1) and similar levels of bulk ZnO (bZnO). The results revealed that soil microbial biomass-C, -N, -P, soil respiration and enzyme activities decreased markedly at higher ZnO levels. The alpha diversity decreased with increasing ZnO level, with more impact under nZnO, while beta diversity analyses indicated a distinct dose- dependent separation of bacterial communities. The dominant taxa including Proteobacteria, Bacterioidetes, Acidobacteria and Planctomycetes significantly increased in abundance, while Firmicutes, Actinobacteria and Chloroflexi decreased in abundance with elevated nZnO and bZnO levels. Redundancy analysis indicated that changes in bacterial community structure instilled a greater dose- rather than size- specific response on key microbial parameters. Predicted key functions did not show a dose- specific response, and at 1000 mg Zn kg−1, methane metabolism as well as starch and sucrose metabolism were attenuated, while functions involving two component systems and bacterial secretion systems were enhanced under bZnO indicating better stress avoidance mechanism than under nZnO. Realtime PCR and microbial endpoint assays confirmed the metagenome derived taxonomic and functional data, respectively. Taxa and functions that varied substantially under stress were established as bioindicators to predict nZnO toxicity in soils. Taxon-function decoupling indicated that the soil bacterial communities deployed adaptive mechanisms under high ZnO, with lesser buffering capacity and resilience of communities under nZnO.
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•Taxon-function decoupling was the bacterial adaptive strategy under high Zn levels.•Soil bacterial communities under nZnO showed lower resilience.•Soil bacterial communities under bZnO showed better Zn stress avoidance.•Nitrospira, Bryobacter, Sphingomonas and Flavisolibacter were the sentinel taxa.