A Poly-γ-glutamate Synthetic System of Bacillus subtilis IFO 3336: Gene Cloning and Biochemical Analysis of Poly-γ-glutamate Produced by Escherichia coli Clone Cells

Three genes encoding a poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-γ-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus ant...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 263; no. 1; pp. 6 - 12
Main Authors:	Ashiuchi, Makoto, Soda, Kenji, Misono, Haruo
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 16-09-1999
Subjects:	Amino Acid Isomerases - genetics Amino Acid Sequence Bacillus anthracis Bacillus natto Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Base Sequence Cloning, Molecular DNA Primers - genetics DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genes, Bacterial Magnesium - pharmacology Manganese - pharmacology Molecular Sequence Data Plasmids - genetics Polyglutamic Acid - biosynthesis Polyglutamic Acid - chemistry Species Specificity Stereoisomerism
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