A Poly-γ-glutamate Synthetic System of Bacillus subtilis IFO 3336: Gene Cloning and Biochemical Analysis of Poly-γ-glutamate Produced by Escherichia coli Clone Cells
Three genes encoding a poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-γ-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus ant...
Saved in:
| Published in: | Biochemical and biophysical research communications Vol. 263; no. 1; pp. 6 - 12 |
|---|---|
| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Elsevier Inc
16-09-1999
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Three genes encoding a poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-γ-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus anthracis. All of pgsB, pgsC, and pgsA genes were essential for the polymer production. Addition of Mn2+, instead of Mg2+, to the polymer-synthesis medium resulted in an increase in the polymer yield. Co-expression of glutamate racemase gene in E. coli cells harboring pgsBCA genes increased both the polymer production and d-glutamate content in the polymer. The polymer produced by the E. coli clone was higher in average molecular size than that produced by B. subtilis IFO 3336. |
|---|---|
| AbstractList | Three genes encoding a poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-gamma-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus anthracis. All of pgsB, pgsC, and pgsA genes were essential for the polymer production. Addition of Mn(2+), instead of Mg(2+), to the polymer-synthesis medium resulted in an increase in the polymer yield. Co-expression of glutamate racemase gene in E. coli cells harboring pgsBCA genes increased both the polymer production and D-glutamate content in the polymer. The polymer produced by the E. coli clone was higher in average molecular size than that produced by B. subtilis IFO 3336. Three genes encoding a poly- gamma -glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly- gamma -glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus anthracis. All of pgsB, pgsC, and pgsA genes were essential for the polymer production. Addition of Mn super(2+), instead of Mg super(2+), to the polymer-synthesis medium resulted in an increase in the polymer yield. Co-expression of glutamate racemase gene in E. coli cells harboring pgsBCA genes increased both the polymer production and -glutamate content in the polymer. The polymer produced by the E. coli clone was higher in average molecular size than that produced by B. subtilis IFO 3336. Three genes encoding a poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E. coli clone produced poly-γ-glutamate extracellularly. The genes, newly designated as pgsBCA, were homologous with capBCA genes of Bacillus anthracis. All of pgsB, pgsC, and pgsA genes were essential for the polymer production. Addition of Mn2+, instead of Mg2+, to the polymer-synthesis medium resulted in an increase in the polymer yield. Co-expression of glutamate racemase gene in E. coli cells harboring pgsBCA genes increased both the polymer production and d-glutamate content in the polymer. The polymer produced by the E. coli clone was higher in average molecular size than that produced by B. subtilis IFO 3336. |
| Author | Ashiuchi, Makoto Misono, Haruo Soda, Kenji |
| Author_xml | – sequence: 1 givenname: Makoto surname: Ashiuchi fullname: Ashiuchi, Makoto organization: Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi, 783-8502, Japan – sequence: 2 givenname: Kenji surname: Soda fullname: Soda, Kenji organization: Department of Biotechnology, Kansai University, Suita, Osaka, 564-8680, Japan – sequence: 3 givenname: Haruo surname: Misono fullname: Misono, Haruo organization: Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi, 783-8502, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10486244$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkcFq3DAQhkVJaTZprz0WnXrzdmTZ8rq3zZKkgUACSaE3IcnjrIospZId8BP1AfoeeaZouzkUQigIRjDffBLzH5EDHzwS8pHBkgGIL1pHs2Rt2y5Z2a7ekAWDFoqSQXVAFpCJomzZj0NylNJPAMYq0b4jh7m9EmVVLcjvNb0Obi4e_xR3bhrVoEakN7Mftzhak29pxIGGnp4oY52bEk2THq2ziV6cXVHOufhKz9Ej3bjgrb-jynf0xAazxcEa5ejaKzenzGfJy6euY-gmgx3VMz1NeShas7WKmuDsX2UWo3PpPXnbK5fww3M9Jt_PTm8334rLq_OLzfqyMLxhY9FBZ1pgBhgXUPGmFliLmnWgoO8bUa0azfKpG15DK5Aj77XuGBqldWN0xY_J5733PoZfE6ZRDjaZ_APlMUxJNgBcVOL_IGs4B1HWGVzuQRNDShF7eR_toOIsGchdhnKXodxlKHcZ5oFPz-ZJD9j9g-9Dy8BqD2BexIPFKJOx6PMWbUQzyi7Y19xPlkmu2Q |
| CitedBy_id | crossref_primary_10_1016_j_progpolymsci_2016_12_003 crossref_primary_10_1271_bbb_65_2007 crossref_primary_10_6090_jarq_46_305 crossref_primary_10_1039_c3np70025a crossref_primary_10_1016_j_jbiosc_2019_05_014 crossref_primary_10_9748_hcs_2015_156 crossref_primary_10_3923_jm_2007_664_670 crossref_primary_10_1139_W09_003 crossref_primary_10_1016_j_ijbiomac_2024_133179 crossref_primary_10_1099_mic_0_000658 crossref_primary_10_1186_s13568_017_0512_0 crossref_primary_10_3390_applmicrobiol3020026 crossref_primary_10_1016_j_mec_2020_e00144 crossref_primary_10_1016_j_biortech_2010_12_065 crossref_primary_10_1128_JB_183_24_7318_7328_2001 crossref_primary_10_1007_s10295_013_1245_y crossref_primary_10_1007_s10529_006_9080_0 crossref_primary_10_1016_S1381_1177_03_00076_6 crossref_primary_10_1046_j_0014_2956_2001_02475_x crossref_primary_10_1111_j_1365_2958_2005_04746_x crossref_primary_10_1021_bi401644s crossref_primary_10_1002_tcr_20061 crossref_primary_10_1128_IAI_01865_06 crossref_primary_10_1016_S1381_1177_03_00087_0 crossref_primary_10_3136_fstr_20_1229 crossref_primary_10_3136_fstr_17_447 crossref_primary_10_6090_jarq_52_249 crossref_primary_10_1002_btpr_417 crossref_primary_10_1080_21505594_2019_1582975 crossref_primary_10_1002_bit_25838 crossref_primary_10_1111_j_1574_6968_2004_tb09621_x crossref_primary_10_1016_j_biortech_2012_11_092 crossref_primary_10_1128_AEM_05827_11 crossref_primary_10_1271_bbb_60108 crossref_primary_10_1186_s12866_018_1205_9 crossref_primary_10_1038_s41428_020_00413_7 crossref_primary_10_1263_jbb_100_443 crossref_primary_10_22207_JPAM_13_2_10 crossref_primary_10_1016_j_biortech_2011_05_057 crossref_primary_10_1111_1750_3841_13540 crossref_primary_10_1128_AEM_70_7_4249_4255_2004 crossref_primary_10_1016_j_heliyon_2021_e07715 crossref_primary_10_1111_j_1365_2958_2006_05179_x crossref_primary_10_1128_JVI_80_8_4079_4087_2006 crossref_primary_10_1128_AEM_02686_06 crossref_primary_10_1007_s00253_005_1982_6 crossref_primary_10_1016_j_molcatb_2005_06_007 crossref_primary_10_1021_bm2008235 crossref_primary_10_1111_1751_7915_12405 crossref_primary_10_1002_cbdv_200900238 crossref_primary_10_1557_adv_2016_150 crossref_primary_10_1046_j_1365_2958_2000_02069_x crossref_primary_10_1007_s12010_016_2376_1 crossref_primary_10_1134_S0003683811040193 crossref_primary_10_1128_AEM_02672_08 crossref_primary_10_1186_s43094_021_00280_w crossref_primary_10_1007_s12010_019_03004_2 crossref_primary_10_3390_ijms18122644 crossref_primary_10_1128_AEM_70_10_6247_6256_2004 crossref_primary_10_1016_j_ijbiomac_2018_02_082 crossref_primary_10_1186_s13568_018_0636_x crossref_primary_10_1016_j_enzmictec_2022_110139 crossref_primary_10_3389_fchem_2023_1158147 crossref_primary_10_1016_j_vaccine_2011_08_036 crossref_primary_10_1111_1462_2920_12613 crossref_primary_10_1016_j_ymben_2023_12_008 crossref_primary_10_1128_AEM_67_2_1004_1007_2001 crossref_primary_10_1016_S0960_8524_01_00074_8 crossref_primary_10_1016_j_ymben_2019_07_009 crossref_primary_10_1098_rstb_2006_1909 crossref_primary_10_1016_S1389_1723_02_80024_X crossref_primary_10_1007_s11426_007_0061_5 crossref_primary_10_1128_JB_185_7_2379_2382_2003 crossref_primary_10_1002_btpr_2118 crossref_primary_10_1016_j_ijbiomac_2014_12_017 crossref_primary_10_1016_j_ijbiomac_2024_130026 crossref_primary_10_1002_btpr_3293 crossref_primary_10_1038_s41579_021_00540_9 crossref_primary_10_1186_s12866_024_03262_z crossref_primary_10_1002_bit_22913 crossref_primary_10_1016_j_biortech_2005_01_033 crossref_primary_10_1007_s00253_006_0479_2 crossref_primary_10_3389_fmicb_2016_01811 crossref_primary_10_1128_AEM_68_7_3575_3581_2002 crossref_primary_10_1007_s10856_012_4639_x crossref_primary_10_1038_nrmicro2354 crossref_primary_10_1021_acs_jafc_2c05572 crossref_primary_10_1002_jsfa_7318 crossref_primary_10_1111_1751_7915_12072 crossref_primary_10_1093_bbb_zbaa037 crossref_primary_10_1111_1751_7915_12075 crossref_primary_10_1016_j_molcatb_2010_07_014 crossref_primary_10_1111_j_1365_2958_2005_05020_x crossref_primary_10_3390_ma17010015 crossref_primary_10_1128_JB_02516_14 crossref_primary_10_1007_s00284_010_9696_0 crossref_primary_10_1128_AEM_00175_14 crossref_primary_10_3390_life3010181 crossref_primary_10_1007_s00262_010_0903_4 crossref_primary_10_1111_jam_14552 crossref_primary_10_1016_j_procbio_2014_12_024 crossref_primary_10_1146_annurev_micro_55_1_647 crossref_primary_10_1016_S1389_1723_00_88949_5 crossref_primary_10_1186_s12934_021_01570_x crossref_primary_10_2298_JSC221116011S crossref_primary_10_1002_cbdv_200900283 crossref_primary_10_1021_acs_orglett_9b01121 crossref_primary_10_1128_JB_187_22_7765_7772_2005 crossref_primary_10_1016_j_scienta_2018_02_031 crossref_primary_10_1128_AEM_00448_11 crossref_primary_10_1007_s12649_023_02299_x crossref_primary_10_1155_2016_3073949 crossref_primary_10_1128_AEM_69_5_2491_2497_2003 crossref_primary_10_1128_AEM_02012_07 crossref_primary_10_2323_jgam_57_35 crossref_primary_10_1016_S0014_5793_01_03132_5 crossref_primary_10_1007_s11274_022_03390_6 crossref_primary_10_1016_j_bej_2015_04_007 crossref_primary_10_1007_s00253_005_1989_z crossref_primary_10_1116_6_0000737 crossref_primary_10_3923_jbs_2006_687_694 crossref_primary_10_3390_ijms23169059 crossref_primary_10_1080_07388550601166458 crossref_primary_10_1128_JB_184_2_337_343_2002 crossref_primary_10_1016_j_biotechadv_2018_05_006 crossref_primary_10_1080_10826068_2021_1941103 crossref_primary_10_1099_mic_0_081448_0 crossref_primary_10_1128_JB_00438_15 crossref_primary_10_1128_JB_01217_13 crossref_primary_10_1016_j_jchromb_2011_03_029 crossref_primary_10_3390_vaccines7040213 |
| Cites_doi | 10.1016/S0922-338X(97)89261-6 10.1073/pnas.86.7.2172 10.1271/bbb.61.1621 10.1021/bi9701078 10.2323/jgam.43.139 10.1016/0378-1119(94)00662-C 10.1128/jb.171.2.722-730.1989 10.1271/bbb.57.1212 10.1128/JB.76.5.499-503.1958 10.1271/bbb.56.1031 10.1099/00221287-14-1-14 10.1271/bbb.60.1239 10.1271/bbb1961.46.2275 10.1271/bbb.63.792 10.1016/S0021-9258(19)44475-X 10.1021/bi00014a017 10.1099/00221287-143-10-3313 10.1016/0141-8130(95)98153-P 10.1016/S0021-9258(18)51281-3 10.1016/0969-806X(95)00009-M 10.1093/oxfordjournals.jbchem.a022055 10.1021/ac60111a017 10.1042/bj0400297 10.1016/S0021-9258(18)50357-4 |
| ContentType | Journal Article |
| Copyright | 1999 Academic Press Copyright 1999 Academic Press. |
| Copyright_xml | – notice: 1999 Academic Press – notice: Copyright 1999 Academic Press. |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QL 7TM 8FD C1K FR3 P64 RC3 7X8 |
| DOI | 10.1006/bbrc.1999.1298 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Bacteriology Abstracts (Microbiology B) Nucleic Acids Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Genetics Abstracts Technology Research Database Bacteriology Abstracts (Microbiology B) Nucleic Acids Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management MEDLINE - Academic |
| DatabaseTitleList | MEDLINE Genetics Abstracts MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Chemistry Biology |
| EISSN | 1090-2104 |
| EndPage | 12 |
| ExternalDocumentID | 10_1006_bbrc_1999_1298 10486244 S0006291X99912986 |
| Genre | Journal Article Comparative Study |
| GroupedDBID | --- --K --M -~X .55 .GJ .~1 0R~ 1B1 1CY 1RT 1~. 1~5 23N 3O- 4.4 457 4G. 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM 9M8 AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABYKQ ACDAQ ACGFO ACGFS ACNCT ACRLP ADBBV ADEZE ADFGL ADIYS ADMUD ADUVX AEFWE AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG COF CS3 D0L DM4 DOVZS EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q G8K HLW HVGLF HZ~ IHE J1W K-O KOM L7B LG5 LX2 M41 MO0 MVM N9A O-L O9- OAUVE OHT OZT P-9 P2P PC. Q38 R2- RNS ROL RPZ SCC SDF SDG SDP SES SPCBC SSU SSZ T5K TWZ UQL WH7 X7M XPP Y6R ZA5 ZGI ZMT ~02 ~G- ~KM AAHBH AAXKI AKRWK CGR CUY CVF ECM EIF NPM .HR AAYJJ AAYXX ABDPE ABEFU ABXDB ACKIV AEBSH AFJKZ CITATION G-2 GBLVA P-8 RIG SBG SEW WUQ XSW ZKB 7QL 7TM 8FD C1K FR3 P64 RC3 7X8 |
| ID | FETCH-LOGICAL-c371t-d0dc901c0136043756e5651d0a0ff76487b17b15735096e3e3fbbd1ecabb7cb43 |
| ISSN | 0006-291X |
| IngestDate | Fri Oct 25 23:43:34 EDT 2024 Fri Oct 25 02:24:29 EDT 2024 Thu Nov 21 23:52:20 EST 2024 Sat Sep 28 07:34:37 EDT 2024 Fri Feb 23 02:31:26 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Language | English |
| License | Copyright 1999 Academic Press. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c371t-d0dc901c0136043756e5651d0a0ff76487b17b15735096e3e3fbbd1ecabb7cb43 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
| PMID | 10486244 |
| PQID | 17330625 |
| PQPubID | 23462 |
| PageCount | 7 |
| ParticipantIDs | proquest_miscellaneous_70036464 proquest_miscellaneous_17330625 crossref_primary_10_1006_bbrc_1999_1298 pubmed_primary_10486244 elsevier_sciencedirect_doi_10_1006_bbrc_1999_1298 |
| PublicationCentury | 1900 |
| PublicationDate | 1999-09-16 |
| PublicationDateYYYYMMDD | 1999-09-16 |
| PublicationDate_xml | – month: 09 year: 1999 text: 1999-09-16 day: 16 |
| PublicationDecade | 1990 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Biochemical and biophysical research communications |
| PublicationTitleAlternate | Biochem Biophys Res Commun |
| PublicationYear | 1999 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Ito, Tanaka, Ohmachi, Asada (RF9) 1996; 60 Leonard, Housewright, Thorne (RF14) 1958; 76 Vietri, Marrero, Hoover, Welkos (RF25) 1995; 152 Guex-Holzer, Tomcsik (RF3) 1956; 14 Choi, Kunioka (RF6) 1995; 46 Cromwick, Gross (RF15) 1995; 17 Hara, Ueda (RF11) 1982; 46 Presecan, Moszer, Boursier, Cruz Ramos, De la Fuente, Hullo, Lelong, Schleich, Sekowska, Song, Villan, Kunst, Danchin, Glaser (RF20) 1997; 143 Stein, Kluge, Vater, Franke, Otto, Wittmann-Liebold (RF23) 1995; 34 Borbely, Nagasaki, Borbely, Fan, Bhogle, Sevoian (RF5) 1994; 41 Makino, Uchida, Terakado, Sasakawa, Yoshikawa (RF21) 1989; 171 Tanaka, Fujita, Takenishi, Taniguchi (RF4) 1997; 84 Nagai, Koguchi, Ito (RF12) 1997; 43 Eveland, Pompliano, Anderson (RF26) 1997; 36 Abe, Ito, Ohmachi, Asada (RF22) 1997; 61 Dubois, Gilles, Hamilton, Rebers, Smith (RF19) 1956; 28 Bovarnick (RF1) 1942; 145 Kubota, Matsunobu, Uotani, Takebe, Satoh, Tanaka, Taniguchi (RF10) 1993; 57 Troy (RF13) 1973; 248 Ashiuchi, Soda, Misono (RF24) 1999; 63 Gardner, Troy (RF16) 1979; 254 Goto, Kunioka (RF8) 1992; 56 Ashiuchi, Tani, Soda, Misono (RF7) 1998; 123 Hanby, Rydon (RF2) 1946; 40 Sambrook, J, Fritsch, E. F, and, Maniatis, T. 1989, Molecular Cloning: A Laboratory Manual, 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. Chung, Niemela, Miller (RF18) 1989; 86 Kubota (10.1006/bbrc.1999.1298_RF10) 1993; 57 Cromwick (10.1006/bbrc.1999.1298_RF15) 1995; 17 Abe (10.1006/bbrc.1999.1298_RF22) 1997; 61 Goto (10.1006/bbrc.1999.1298_RF8) 1992; 56 Nagai (10.1006/bbrc.1999.1298_RF12) 1997; 43 10.1006/bbrc.1999.1298_RF17 Makino (10.1006/bbrc.1999.1298_RF21) 1989; 171 Vietri (10.1006/bbrc.1999.1298_RF25) 1995; 152 Presecan (10.1006/bbrc.1999.1298_RF20) 1997; 143 Gardner (10.1006/bbrc.1999.1298_RF16) 1979; 254 Ashiuchi (10.1006/bbrc.1999.1298_RF24) 1999; 63 Bovarnick (10.1006/bbrc.1999.1298_RF1) 1942; 145 Ashiuchi (10.1006/bbrc.1999.1298_RF7) 1998; 123 Troy (10.1006/bbrc.1999.1298_RF13) 1973; 248 Dubois (10.1006/bbrc.1999.1298_RF19) 1956; 28 Leonard (10.1006/bbrc.1999.1298_RF14) 1958; 76 Stein (10.1006/bbrc.1999.1298_RF23) 1995; 34 Guex-Holzer (10.1006/bbrc.1999.1298_RF3) 1956; 14 Eveland (10.1006/bbrc.1999.1298_RF26) 1997; 36 Ito (10.1006/bbrc.1999.1298_RF9) 1996; 60 Hara (10.1006/bbrc.1999.1298_RF11) 1982; 46 Hanby (10.1006/bbrc.1999.1298_RF2) 1946; 40 Chung (10.1006/bbrc.1999.1298_RF18) 1989; 86 Tanaka (10.1006/bbrc.1999.1298_RF4) 1997; 84 Choi (10.1006/bbrc.1999.1298_RF6) 1995; 46 Borbely (10.1006/bbrc.1999.1298_RF5) 1994; 41 |
| References_xml | – volume: 46 start-page: 2275 year: 1982 end-page: 2281 ident: RF11 publication-title: Agric. Biol. Chem. contributor: fullname: Ueda – volume: 171 start-page: 722 year: 1989 end-page: 730 ident: RF21 publication-title: J. Bacteriol. contributor: fullname: Yoshikawa – volume: 36 start-page: 6223 year: 1997 end-page: 6229 ident: RF26 publication-title: Biochemistry contributor: fullname: Anderson – volume: 34 start-page: 4633 year: 1995 end-page: 4642 ident: RF23 publication-title: Biochemistry contributor: fullname: Wittmann-Liebold – volume: 76 start-page: 499 year: 1958 end-page: 503 ident: RF14 publication-title: J. Bacteriol. contributor: fullname: Thorne – volume: 14 start-page: 14 year: 1956 end-page: 25 ident: RF3 publication-title: J. Gen. Microbiol. contributor: fullname: Tomcsik – volume: 56 start-page: 1031 year: 1992 end-page: 1035 ident: RF8 publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Kunioka – volume: 43 start-page: 139 year: 1997 end-page: 143 ident: RF12 publication-title: J. Gen. Appl. Microbiol. contributor: fullname: Ito – volume: 123 start-page: 1156 year: 1998 end-page: 1163 ident: RF7 publication-title: J. Biochem. contributor: fullname: Misono – volume: 60 start-page: 1239 year: 1996 end-page: 1242 ident: RF9 publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Asada – volume: 61 start-page: 1621 year: 1997 end-page: 1625 ident: RF22 publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Asada – volume: 145 start-page: 415 year: 1942 end-page: 424 ident: RF1 publication-title: J. Biol. Chem. contributor: fullname: Bovarnick – volume: 57 start-page: 1212 year: 1993 end-page: 1213 ident: RF10 publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Taniguchi – volume: 86 start-page: 2172 year: 1989 end-page: 2175 ident: RF18 publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Miller – volume: 41 start-page: 1077 year: 1994 ident: RF5 publication-title: Polym. Bull. contributor: fullname: Sevoian – volume: 17 start-page: 259 year: 1995 end-page: 267 ident: RF15 publication-title: Int. J. Biol. Macromol. contributor: fullname: Gross – volume: 254 start-page: 6262 year: 1979 end-page: 6269 ident: RF16 publication-title: J. Biol. Chem. contributor: fullname: Troy – volume: 84 start-page: 361 year: 1997 end-page: 364 ident: RF4 publication-title: J. Ferment. Bioeng. contributor: fullname: Taniguchi – volume: 248 start-page: 305 year: 1973 end-page: 315 ident: RF13 publication-title: J. Biol. Chem. contributor: fullname: Troy – volume: 28 start-page: 350 year: 1956 end-page: 356 ident: RF19 publication-title: Anal. Chem. contributor: fullname: Smith – volume: 143 start-page: 3313 year: 1997 end-page: 3328 ident: RF20 publication-title: Microbiology contributor: fullname: Glaser – volume: 40 start-page: 297 year: 1946 end-page: 309 ident: RF2 publication-title: Biochem. J. contributor: fullname: Rydon – volume: 152 start-page: 1 year: 1995 end-page: 9 ident: RF25 publication-title: Gene contributor: fullname: Welkos – volume: 63 start-page: 792 year: 1999 end-page: 798 ident: RF24 publication-title: Biosci. Biotechnol. Biochem. contributor: fullname: Misono – volume: 46 start-page: 175 year: 1995 end-page: 179 ident: RF6 publication-title: Radiat. Phys. Chem. contributor: fullname: Kunioka – volume: 41 start-page: 1077 year: 1994 ident: 10.1006/bbrc.1999.1298_RF5 publication-title: Polym. Bull. contributor: fullname: Borbely – volume: 84 start-page: 361 year: 1997 ident: 10.1006/bbrc.1999.1298_RF4 publication-title: J. Ferment. Bioeng. doi: 10.1016/S0922-338X(97)89261-6 contributor: fullname: Tanaka – volume: 86 start-page: 2172 year: 1989 ident: 10.1006/bbrc.1999.1298_RF18 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.86.7.2172 contributor: fullname: Chung – volume: 61 start-page: 1621 year: 1997 ident: 10.1006/bbrc.1999.1298_RF22 publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.61.1621 contributor: fullname: Abe – volume: 36 start-page: 6223 year: 1997 ident: 10.1006/bbrc.1999.1298_RF26 publication-title: Biochemistry doi: 10.1021/bi9701078 contributor: fullname: Eveland – volume: 43 start-page: 139 year: 1997 ident: 10.1006/bbrc.1999.1298_RF12 publication-title: J. Gen. Appl. Microbiol. doi: 10.2323/jgam.43.139 contributor: fullname: Nagai – volume: 152 start-page: 1 year: 1995 ident: 10.1006/bbrc.1999.1298_RF25 publication-title: Gene doi: 10.1016/0378-1119(94)00662-C contributor: fullname: Vietri – volume: 171 start-page: 722 year: 1989 ident: 10.1006/bbrc.1999.1298_RF21 publication-title: J. Bacteriol. doi: 10.1128/jb.171.2.722-730.1989 contributor: fullname: Makino – volume: 57 start-page: 1212 year: 1993 ident: 10.1006/bbrc.1999.1298_RF10 publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.57.1212 contributor: fullname: Kubota – volume: 76 start-page: 499 year: 1958 ident: 10.1006/bbrc.1999.1298_RF14 publication-title: J. Bacteriol. doi: 10.1128/JB.76.5.499-503.1958 contributor: fullname: Leonard – volume: 56 start-page: 1031 year: 1992 ident: 10.1006/bbrc.1999.1298_RF8 publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.56.1031 contributor: fullname: Goto – ident: 10.1006/bbrc.1999.1298_RF17 – volume: 14 start-page: 14 year: 1956 ident: 10.1006/bbrc.1999.1298_RF3 publication-title: J. Gen. Microbiol. doi: 10.1099/00221287-14-1-14 contributor: fullname: Guex-Holzer – volume: 60 start-page: 1239 year: 1996 ident: 10.1006/bbrc.1999.1298_RF9 publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.60.1239 contributor: fullname: Ito – volume: 46 start-page: 2275 year: 1982 ident: 10.1006/bbrc.1999.1298_RF11 publication-title: Agric. Biol. Chem. doi: 10.1271/bbb1961.46.2275 contributor: fullname: Hara – volume: 63 start-page: 792 year: 1999 ident: 10.1006/bbrc.1999.1298_RF24 publication-title: Biosci. Biotechnol. Biochem. doi: 10.1271/bbb.63.792 contributor: fullname: Ashiuchi – volume: 248 start-page: 305 year: 1973 ident: 10.1006/bbrc.1999.1298_RF13 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)44475-X contributor: fullname: Troy – volume: 34 start-page: 4633 year: 1995 ident: 10.1006/bbrc.1999.1298_RF23 publication-title: Biochemistry doi: 10.1021/bi00014a017 contributor: fullname: Stein – volume: 143 start-page: 3313 year: 1997 ident: 10.1006/bbrc.1999.1298_RF20 publication-title: Microbiology doi: 10.1099/00221287-143-10-3313 contributor: fullname: Presecan – volume: 17 start-page: 259 year: 1995 ident: 10.1006/bbrc.1999.1298_RF15 publication-title: Int. J. Biol. Macromol. doi: 10.1016/0141-8130(95)98153-P contributor: fullname: Cromwick – volume: 145 start-page: 415 year: 1942 ident: 10.1006/bbrc.1999.1298_RF1 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)51281-3 contributor: fullname: Bovarnick – volume: 46 start-page: 175 year: 1995 ident: 10.1006/bbrc.1999.1298_RF6 publication-title: Radiat. Phys. Chem. doi: 10.1016/0969-806X(95)00009-M contributor: fullname: Choi – volume: 123 start-page: 1156 year: 1998 ident: 10.1006/bbrc.1999.1298_RF7 publication-title: J. Biochem. doi: 10.1093/oxfordjournals.jbchem.a022055 contributor: fullname: Ashiuchi – volume: 28 start-page: 350 year: 1956 ident: 10.1006/bbrc.1999.1298_RF19 publication-title: Anal. Chem. doi: 10.1021/ac60111a017 contributor: fullname: Dubois – volume: 40 start-page: 297 year: 1946 ident: 10.1006/bbrc.1999.1298_RF2 publication-title: Biochem. J. doi: 10.1042/bj0400297 contributor: fullname: Hanby – volume: 254 start-page: 6262 year: 1979 ident: 10.1006/bbrc.1999.1298_RF16 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)50357-4 contributor: fullname: Gardner |
| SSID | ssj0011469 |
| Score | 2.0707717 |
| Snippet | Three genes encoding a poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The E.... Three genes encoding a poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli. The... Three genes encoding a poly- gamma -glutamate synthetic system of Bacillus subtilis IFO 3336 (Bacillus natto) were cloned and expressed in Escherichia coli.... |
| SourceID | proquest crossref pubmed elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 6 |
| SubjectTerms | Amino Acid Isomerases - genetics Amino Acid Sequence Bacillus anthracis Bacillus natto Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Base Sequence Cloning, Molecular DNA Primers - genetics DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genes, Bacterial Magnesium - pharmacology Manganese - pharmacology Molecular Sequence Data Plasmids - genetics Polyglutamic Acid - biosynthesis Polyglutamic Acid - chemistry Species Specificity Stereoisomerism |
| Title | A Poly-γ-glutamate Synthetic System of Bacillus subtilis IFO 3336: Gene Cloning and Biochemical Analysis of Poly-γ-glutamate Produced by Escherichia coli Clone Cells |
| URI | https://dx.doi.org/10.1006/bbrc.1999.1298 https://www.ncbi.nlm.nih.gov/pubmed/10486244 https://search.proquest.com/docview/17330625 https://search.proquest.com/docview/70036464 |
| Volume | 263 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3NjtMwELa6u0JwQbDLT_n1gYVDlCWJ88utLVl1OQDSLtLeothxRSA0qyY59Il4AN6DZ2ImjtNUSyVAQqqiKEo9luaL_Xk885mQF2LhO9KKMjPinmPiFGByR3imFCEHdiw8kWIccn4evL8M38ZuPBrpUxA2z_6rp-EZ-BorZ__C232j8ADuwedwBa_D9Y_8PsGUtrV5PIuPpwwz0usUSKk0ztdL4Hooz6pEypElTlORF0VTGVXD67zIK-Ps9IPBGPMxUICK1MasUPFajK9PczxeS-kLDMVMfm_wY6slqwhuXCE2csyrNgB5edssNC6LotraVh5YQIs8L680kDpZIkyUH9S09EuCSfU5x4NdVAHS17Iu--BRmaVdCdKXvAcYZlG1YeJ5umrKTfBDCSZEpqrNVBE5XZWzlTSKU7DpRO1RPDDHqYHdiizT6Y461iO_042tQ4ircdwfEAKV5n1tqgEjgAXOVwIrPqMTIE7hZlLtUx3PsTfYGWTi8Iq_Rw4cGBRhTD6YnMWX7_o9L5izusWa6r2WGLX819tWdlGoXUuklipd3CG3uzUOnShw3iUjuTwkR4CYuvy2pi9pm3XcbucckhtTfXdzps8ePCLfJ7QF1c8fG0DRHsFUIZiWC6oRTDWCKSCYIoLfUMQv7fBLAU10gC6q8YuNXDelsUv5mg6wSxG7bZPQMGL3Hvl0Gl_M5mZ3pIgpWGDXZmZlAhiwQKVCVPXyfAkrGjuzUmuxCHxYvXMbfl7AUBZJMskWnGe2FCnngeAuu0_2l2DkIaFAjEMHZj-W2tINIxFaPpdpwNIoBWLo8TF5pb2UXCnlmERphPsJ-jNBfybozzGxtROTjvcqPpsA1nb-57n2dgK-wV2-dCnLpkrsgDFAnLf7jQBFqFzfHZMHCiaD_rlYMOY--ocePSa3Nl_oE7Jfrxr5lOxVWfOsQ_ovMnPwaw |
| link.rule.ids | 315,782,786,27933,27934 |
| linkProvider | Elsevier |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Poly-%CE%B3-glutamate+Synthetic+System+of+Bacillus+subtilis+IFO+3336%3A+Gene+Cloning+and+Biochemical+Analysis+of+Poly-%CE%B3-glutamate+Produced+by+Escherichia+coli+Clone+Cells&rft.jtitle=Biochemical+and+biophysical+research+communications&rft.au=Ashiuchi%2C+Makoto&rft.au=Soda%2C+Kenji&rft.au=Misono%2C+Haruo&rft.date=1999-09-16&rft.pub=Elsevier+Inc&rft.issn=0006-291X&rft.eissn=1090-2104&rft.volume=263&rft.issue=1&rft.spage=6&rft.epage=12&rft_id=info:doi/10.1006%2Fbbrc.1999.1298&rft.externalDocID=S0006291X99912986 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-291X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-291X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-291X&client=summon |