Neonicotinoid effects on tropical bees: Imidacloprid impairs innate appetitive responsiveness, learning and memory in the stingless bee Melipona quadrifasciata

• Published in: The Science of the total environment Vol. 877; p. 162859
• Main Authors: Aguiar, João Marcelo Robazzi Bignelli Valente, Nocelli, Roberta Cornélio Ferreira, Giurfa, Martin, Nascimento, Fábio Santos
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-06-2023
• Subjects: Africanized honey bees; Animals; Appetitive responsiveness; Bees; Learning; Neonicotinoids - toxicity; Nitro Compounds - toxicity; Pesticides; Pollinators; Stingless bees; Appetitive responsiveness; Stingless bees; Africanized honey bees; Pesticides; Pollinators
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.162859

Together with other anthropogenic factors, pesticides play a major role in pollinator decline worldwide. Most studies on their influence on pollinators have focused on honey bees given the suitability of this insect for controlled behavioral testing and raising. Yet, studies on pesticide impact should also contemplate tropical species, which contribute a major part of biodiversity and which have remained so far neglected. Here we focused on the stingless bee Melipona quadrifasciata and asked if the widely used neonicotinoid imidacloprid disrupts its learning and memory capabilities. We fed stingless bees with 0.1, 0.5 or 1 ng of imidacloprid, tested their innate appetitive responsiveness and trained them to associate odors and sucrose reward using the olfactory conditioning of the proboscis extension response. The same experiments were performed on Africanized honey bees. One hour after intoxication, both species decreased their innate responsiveness to sucrose but the effect was more accentuated in stingless bees. In both species, learning and memory were affected in a dose-dependent manner. These results indicate that pesticides have dramatic consequences on tropical bee species and claim for rational policies regulating their use in the tropics.
[Display omitted]
•The effect of imidacloprid was studied in two tropical bee species from Brazil.•In both species, imidacloprid disrupted innate responsiveness to sucrose solution.•This effect was more accentuated in stingless bees than in Africanized honey bees.•Both species exhibited dose-dependent impairment of learning and memory.•Imidacloprid thus impacts negatively innate and plastic behaviors of tropical bees.