rolB Gene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

• Published in: Plant physiology (Bethesda) Vol. 158; no. 3; pp. 1371 - 1381
• Main Authors: Bulgakov, Victor P, Gorpenchenko, Tatiana Y, Veremeichik, Galina N, Shkryl, Yuri N, Tchernoded, Galina K, Bulgakov, Dmitry V, Aminin, Dmitry L, Zhuravlev, Yuri N
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01-03-2012
• Subjects: Agrobacterium - genetics; Agrobacterium rhizogenes; Antioxidants; Antioxidants - metabolism; Arabidopsis - cytology; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; ascorbate peroxidase; Ascorbate Peroxidases - genetics; Ascorbate Peroxidases - metabolism; B lymphocytes; bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Callus; catalase; Cell culture techniques; Cell Death; cell differentiation; Cell Survival; Culture Media - metabolism; Cultured cells; gene expression; Genes; Glutathione - metabolism; Light; loci; menadione; metabolism; microscopy; oncogenes; Oxidative Stress; Panax - cytology; Panax - drug effects; Panax - genetics; Panax - metabolism; Panax ginseng; paraquat; Paraquat - pharmacology; Plant cells; Plant Cells - drug effects; Plant Cells - metabolism; Plants; PLANTS INTERACTING WITH OTHER ORGANISMS; Plants, Genetically Modified - cytology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rhizobium rhizogenes; rooting; roots; Rubia; Rubia - drug effects; Rubia - genetics; Rubia - metabolism; Rubia cordifolia; Salt-Tolerant Plants - cytology; Salt-Tolerant Plants - drug effects; Salt-Tolerant Plants - genetics; Salt-Tolerant Plants - metabolism; Sodium Chloride - pharmacology; superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Transformed cell line; Vitamin K 3 - pharmacology
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.111.191494

The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.