A 16-kDa Fragment of Collagen Type XIV Is a Novel Neutrophil Chemotactic Factor Purified from Rat Granulation Tissue

• Published in: Biochemical and biophysical research communications Vol. 256; no. 3; pp. 642 - 645
• Main Authors: Nakagawa, Hideo, Takano, Katsuhiko, Kuzumaki, Hidekazu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24-03-1999
• Subjects: Amino Acid Sequence; Animals; Binding Sites; Calcium - metabolism; Chemotactic Factors - chemistry; Chemotactic Factors - isolation & purification; Chemotactic Factors - metabolism; Chemotactic Factors - pharmacology; Chemotaxis, Leukocyte; Chromatography, Affinity; Chromatography, High Pressure Liquid; Collagen - chemistry; Collagen - isolation & purification; Collagen - pharmacology; Dose-Response Relationship, Drug; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Glycoproteins - pharmacology; Granulation Tissue - chemistry; Heparin - metabolism; Male; Molecular Sequence Data; Molecular Weight; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - physiology; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Peptide Fragments - pharmacology; Rats; Rats, Wistar; Sequence Analysis; Serine Endopeptidases - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1999.0393

A neutrophil chemotactic factor has been purified from the homogenate of rat granulation tissues. The purified chemoattractant was a basic protein with heparin-binding site and gave a single band corresponding to a molecular mass of 16 kDa on SDS–PAGE under reducing conditions. The chemoattractant was treated with lysylendopeptidase and the resulting peptides were isolated by reversed-phase HPLC. Amino acid sequences of the peptides were almost identical with the sequence of N-terminal fibronectin type III domain of human collagen type XIV, suggesting that the purified chemoattractant consists mainly of N-terminal fibronectin type III domain and the adjacent heparin-binding site of rat collagen type XIV. The 16-kDa fragment of collagen type XIV dose dependently attracted rat neutrophils and transiently increased the intracellular free Ca2+concentration of neutrophils. The results suggest that the novel chemoattractant plays a role in neutrophil recruitment in rat inflammation.